Song Dongcheol, Lee Jihwan, Kwak Woogi, Oh Hanjin, Chang Seyeon, An Jaewoo, Cho Hyunah, Park Sehyun, Jeon Kyeongho, Cho Jinho
Department of Animal Science, Chungbuk National University, Cheongju, Republic of Korea.
Department of Poultry Science, University of Georgia (UGA), Athens, GA, United States.
Front Vet Sci. 2023 Jul 19;10:1187002. doi: 10.3389/fvets.2023.1187002. eCollection 2023.
In order to make piglet diets more effective, it is necessary to investigate effective methods for breaking down xylan in cereal. The objective of this study was to determine the effects of dietary stimbiotic (STB) supplementation on growth performance, intestinal morphology, immune response and intestinal microbiota in weaned piglets. A total of 24 (Duroc × Yorkshire × Landrace) weaned pigs (initial body weight of 8.01 ± 0.38 kg and 28 ± 3 d old), were assigned to 4 treatments with 6 replicates per treatment. Pigs were housed in individual pens for 17 days, including 5 days adaption period and 12 days after the first challenge. The experiment was conducted in a 2 × 2 factorial arrangement of treatments consisting of two levels of challenge (challenge and non-challenge) and two levels of STB (0 and 0.5 g/kg diet). Supplementations of STB 0.5 g/kg improved the gain to feed ratio (G:F) ( < 0.05) in piglets challenged with shiga toxigenic (STEC). STB supplementation decreased ( < 0.05) white blood cells, neutrophils, lymphocytes, and expression levels of tumor necrosis factor-alpha and interleukin-6. Supplementation of STB improved ( < 0.05) the lymphocytes and neutrophils in piglets challenged with STEC on 12 dpi. Supplementation of STB also improved ( < 0.05) the villus height to-crypt depth ratio of ileum in piglets challenged with STEC. Supplementation of STB increased ( < 0.05) the expression levels of claudin-1 of ileum. In genus level, supplementation of STB increased ( < 0.001) the abundance of compared to non-supplementation of STB groups in pre-inoculation period. Also, supplementation of STB decreased ( < 0.05) the abundance of and _group compared to non-supplementation of STB groups in post-inoculation period. In phylum level, supplementation of STB increased ( < 0.05) the abundance of and in pre-inoculation period. challenge increased the abundance of compared to non-challenged group in post-inoculation period. In conclusion, these findings indicated that STB supplementation could alleviate a decrease of the performance, immune response, and inflammatory response in piglets induced by the STEC challenge.
为了使仔猪日粮更有效，有必要研究分解谷物中木聚糖的有效方法。本研究的目的是确定日粮添加合生元（STB）对断奶仔猪生长性能、肠道形态、免疫反应和肠道微生物群的影响。总共24头（杜洛克×约克夏×长白）断奶仔猪（初始体重8.01±0.38千克，28±3日龄），被分配到4个处理组，每个处理组6个重复。仔猪在个体栏中饲养17天，包括5天适应期和首次攻毒后12天。实验采用2×2析因设计，包括两个攻毒水平（攻毒和未攻毒）和两个STB水平（0和0.5克/千克日粮）。添加0.5克/千克STB提高了感染产志贺毒素大肠杆菌（STEC）的仔猪的料重比（G:F）（P<0.05）。添加STB降低了（P<0.05）白细胞、中性粒细胞、淋巴细胞以及肿瘤坏死因子-α和白细胞介素-6的表达水平。在攻毒后12天，添加STB提高了（P<0.05）感染STEC的仔猪的淋巴细胞和中性粒细胞数量。添加STB还提高了（P<0.05）感染STEC的仔猪回肠绒毛高度与隐窝深度的比值。添加STB增加了（P<0.05）回肠紧密连接蛋白-1的表达水平。在属水平上，与未添加STB组相比，添加STB在接种前增加了（P<0.001）[此处原文缺失具体菌属名称]的丰度。此外，与未添加STB组相比，添加STB在接种后降低了（P<0.05）[此处原文缺失具体菌属名称]和[此处原文缺失具体菌属名称]组的丰度。在门水平上，添加STB在接种前增加了（P<0.05）[此处原文缺失具体菌门名称]和[此处原文缺失具体菌门名称]的丰度。与未攻毒组相比，攻毒在接种后增加了[此处原文缺失具体菌门名称]的丰度。总之，这些结果表明，添加STB可以缓解STEC攻毒诱导的仔猪性能、免疫反应和炎症反应的下降。
