Afsharyan Nazanin P, Sannemann Wiebke, Ballvora Agim, Léon Jens
Institute for Crop Science and Resource Conservation, Chair of Plant Breeding University of Bonn Bonn Germany.
Department of Plant Breeding Justus Liebig University Giessen Giessen Germany.
Plant Direct. 2023 Aug 2;7(8):e516. doi: 10.1002/pld3.516. eCollection 2023 Aug.
Barley is the fourth most cultivated cereal worldwide, and drought is a major cause of its yield loss by negatively affecting its development. Hence, better understanding developmental mechanisms that control complex polygenic yield-related traits under drought is essential to uncover favorable yield regulators. This study evaluated seven above-ground yield-related traits under well-watered (WW) and late-terminal drought (TD) treatment using 534 spring barley multiparent advanced generation intercross double haploid (DH) lines. The analysis of quantitative trait loci (QTL) for WW, TD, marker by treatment interaction, and drought stress tolerance identified 69, 64, 25, and 25 loci, respectively, for seven traits from which 15 loci were common for at least three traits and 17 were shared by TD and drought stress tolerance. Evaluation of allelic effects for a QTL revealed varying effect of parental alleles. Results showed prominent QTL located on major flowering time gene with favorable effects for grain weight under TD when flowering time was not significantly affected, suggesting that this gene might be linked with increasing grain weight by ways other than timing of flowering under late-terminal drought stress. Furthermore, a desirable novel QTL allele was identified on chromosome 5H for grain number under TD nearby sucrose transporter gene . The findings indicated that spring barley multiparent advanced generation intercross population can provide insights to improve yield under complex condition of drought.
大麦是全球种植面积第四大的谷类作物，干旱是导致其产量损失的主要原因，因为干旱会对其生长发育产生负面影响。因此，深入了解干旱条件下控制复杂多基因产量相关性状的发育机制对于发现有利的产量调控因子至关重要。本研究使用534个春大麦多亲本高世代杂交双单倍体（DH）株系，在充分供水（WW）和后期终端干旱（TD）处理条件下，对7个地上部产量相关性状进行了评估。对WW、TD、处理互作标记以及干旱胁迫耐受性的数量性状位点（QTL）分析分别鉴定出69、64、25和25个与7个性状相关的位点，其中15个位点至少在三个性状中是共同的，17个位点在TD和干旱胁迫耐受性中是共享的。对一个QTL的等位基因效应评估显示亲本等位基因的效应各不相同。结果表明，位于主要开花时间基因上的显著QTL在开花时间未受到显著影响时，对TD条件下的粒重具有有利影响，这表明该基因可能通过后期终端干旱胁迫下开花时间以外的方式与增加粒重相关。此外，在5H染色体上靠近蔗糖转运蛋白基因的位置，鉴定出一个在TD条件下与粒数相关的理想新QTL等位基因。研究结果表明，春大麦多亲本高世代杂交群体可为在复杂干旱条件下提高产量提供见解。
