Oso Babatunde, Agboola Olubukola, Olaoye Ige
Department of Biochemistry, McPherson University, Seriki Sotayo, Ogun State, Nigeria.
Avicenna J Med Biotechnol. 2023 Jul-Sep;15(3):180-187. doi: 10.18502/ajmb.v15i3.12928.
has been used in traditional medicine for the management of complications associated with diabetes mellitus. Several phytochemicals with different pharmacological properties have been previously identified from the botanical; however, the mechanisms of actions of this plant vis-à-vis inhibition of non-enzymatic protein glycation are not known. This study aimed at understanding the putative mechanisms underlying the antiglycation properties of extracts experimental and theoretical approaches.
The antiglycation properties of the plant were evaluated by studying the inhibitory actions of methanol and aqueous extracts on glucose-induced glycation of Bovine Serum Albumin (BSA) and protein aggregation. The mode of binding of identified phenolics of the botanical with BSA, amyloid beta-peptide (1-42) and 3D amyloid beta (1-42) fibrils were also investigated.
The experimental properties of the extracts showed that the extracts could prevent inductions of protein glycation and protein folding. The molecular docking analyses revealed that phenolics had better binding affinities with chlorogenic acid showing the highest binding score (-7.13±0.04 ) towards BSA than glucose and their respective interactions with BSA could prevent glucose-induced protein aggregation.
Consequently, the results of this study provide insight into the probable mechanisms of actions of the extracts of against the inhibition of advanced glycation end products formation.
已被用于传统医学中治疗与糖尿病相关的并发症。此前已从该植物中鉴定出几种具有不同药理特性的植物化学物质；然而，该植物相对于抑制非酶蛋白糖基化的作用机制尚不清楚。本研究旨在通过实验和理论方法了解提取物抗糖基化特性的潜在机制。
通过研究甲醇提取物和水提取物对葡萄糖诱导的牛血清白蛋白（BSA）糖基化和蛋白质聚集的抑制作用，评估该植物的抗糖基化特性。还研究了该植物中已鉴定酚类物质与BSA、淀粉样β肽（1-42）和3D淀粉样β（1-42）纤维的结合模式。
提取物的实验特性表明，提取物可以防止蛋白质糖基化和蛋白质折叠的诱导。分子对接分析显示，酚类物质与绿原酸具有更好的结合亲和力，绿原酸对BSA的结合分数最高（-7.13±0.04），高于葡萄糖，且它们与BSA的各自相互作用可以防止葡萄糖诱导的蛋白质聚集。
因此，本研究结果为该植物提取物抑制晚期糖基化终产物形成的可能作用机制提供了见解。
