Department of Toxicology, Faculty of Pharmacy, Wroclaw Medical University, Borowska Str. 211, 50-556 Wrocław, Poland.
Department of Pharmacognosy and Herbal Medicines, Faculty of Pharmacy, Wroclaw Medical University, Borowska Str. 211A, 50-556 Wrocław, Poland.
Molecules. 2019 Jul 24;24(15):2689. doi: 10.3390/molecules24152689.
Hyperglycemia and oxidative stress may be observed in different diseases as important factors connected with their development. They often occur simultaneously and are considered together as one process: Glycoxidation. This can influence the function or structure of many macromolecules, for example albumin, by changing their physiological properties. This disturbs the homeostasis of the organism, so the search for natural compounds able to inhibit the glycoxidation process is a current and important issue. The aim of this study was the examination of the antiglycoxidative capacity of 16 selected phenolic compounds, belonging to three phenolic groups, as potential therapeutic agents. Their antiglycoxidative ability, in two concentrations (2 and 20 µM), were examined by in vitro study. The inhibition of the formation of both glycoxidative products (advanced glycation end products (AGEs) and advanced oxidation protein products (AOPPs)) were assayed. Stronger antiglycoxidative action toward the formation of both AOPPs and AGEs was observed for homoprotocatechuic and ferulic acids in lower concentrations, as well as catechin, quercetin, and 8--methylurolithin A in higher concentrations. Homoprotocatechuic acid demonstrated the highest antiglycoxidative capacity in both examined concentrations and amongst all of them. A strong, significant correlation between the percentage of AOPPs and AGEs inhibition by compounds from all phenolic groups, in both examined concentrations, was observed. The obtained results give an insight into the antiglycoxidative potential of phenolic compounds and indicate homoprotocatechuic acid to be the most promising antiglycoxidative agent, but further biological and pharmacological studies are needed.
高血糖和氧化应激可能在不同疾病中作为与其发展相关的重要因素而被观察到。它们经常同时发生，并被视为一个过程：糖基化。这会影响许多大分子的功能或结构，例如白蛋白，通过改变其生理特性。这会扰乱机体的内稳态，因此寻找能够抑制糖基化过程的天然化合物是当前的一个重要问题。本研究的目的是研究 16 种选定的酚类化合物的抗糖基化能力，这些化合物属于三个酚类群组，作为潜在的治疗剂。通过体外研究，在两种浓度（2 和 20 µM）下检查了它们的抗糖基化能力。测定了对两种糖基化产物（晚期糖基化终产物（AGEs）和晚期氧化蛋白产物（AOPPs））形成的抑制作用。在较低浓度下，对 HOMOPROTOTATECHUIC 和阿魏酸以及儿茶素、槲皮素和 8--甲基尿烷 A 在较高浓度下，对两种 AOPPs 和 AGEs 的形成均表现出更强的抗糖基化作用。在两种检查浓度下，均显示 HOMOPROTOTATECHUIC 具有最高的抗糖基化能力，在所有研究的化合物中也是如此。在两种检查浓度下，观察到来自所有酚类群组的化合物对 AOPPs 和 AGEs 抑制的百分比之间存在很强的显著相关性。研究结果深入了解了酚类化合物的抗糖基化潜力，并表明 HOMOPROTOTATECHUIC 是最有前途的抗糖基化剂，但需要进一步的生物学和药理学研究。
