急性髓系白血病和急性淋巴细胞白血病中胎盘特异性 1（PLAC1）表达谱的研究

Investigation of Expression Profile of Placenta-specific 1 (PLAC1) in Acute Myeloid and Lymphoid Leukemias.

作者信息

Gholami Parastou, Asgarian-Omran Hossein, Yaghmaie Marjan, Mahmudian Jafar, Kianersi Shirin, Salari Sina, Zaboli Ehsan, Jeddi-Tehrani Mahmood, Zarnani Amir-Hassan, Shabani Mahdi

机构信息

Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.

出版信息

Avicenna J Med Biotechnol. 2023 Jul-Sep;15(3):167-172. doi: 10.18502/ajmb.v15i3.12926.

DOI:10.18502/ajmb.v15i3.12926

PMID:37538244

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10395456/

Abstract

BACKGROUND

Placenta-specific 1 (PLAC1) is one of the cancer-testis-placenta antigens that has no expression in normal tissue except placenta trophoblast and testicular germ cells, but is overexpressed in a variety of solid tumors. There is a lack of studies on the expression of PLAC1 in leukemia. We investigated expression of in Acute Myeloid Leukemia (AML) and Acute Lymphoblastic Leukemia (ALL).

METHODS

In this study, we investigated expression pattern of gene in peripheral blood and bone marrow mononuclear cells of newly-diagnosed patients with AML (n=31) and ALL (n=31) using quantitative real-time PCR. Normal subjects (n=17) were considered as control. The PLAC1 protein expression in the samples were also detected using western blotting.

RESULTS

Our data demonstrated that transcripts had 2.7 and 2.9 fold-change increase in AML and ALL, respectively, compared to normal samples. transcript expression was totally negative in all studied normal subjects. Level of mRNA expression in ALL statistically increased compared to normal samples (p=0.038). However, relative mRNA expression of in AML was not significant in comparison to normal subjects (p=0.848). Furthermore, relative mRNA expression of in AML subtypes was not statistically significant (p=0.756). gene expression showed no difference in demographical clinical and para-clinical parameters. Western blotting confirmed expression of PLAC1 in the ALL and AML samples.

CONCLUSION

Considering expression profile in acute leukemia, PLAC1 could be a potential marker in leukemia which needs complementary studies in the future.

摘要

背景

胎盘特异性1（PLAC1）是癌-睾丸-胎盘抗原之一，除胎盘滋养层和睾丸生殖细胞外，在正常组织中无表达，但在多种实体瘤中过表达。目前缺乏关于PLAC1在白血病中表达的研究。我们调查了PLAC1在急性髓系白血病（AML）和急性淋巴细胞白血病（ALL）中的表达情况。

方法

在本研究中，我们使用定量实时PCR研究了31例新诊断的AML患者和31例ALL患者外周血和骨髓单个核细胞中PLAC1基因的表达模式。17名正常受试者作为对照。还使用蛋白质印迹法检测了样本中PLAC1蛋白的表达。

结果

我们的数据表明，与正常样本相比，AML和ALL中PLAC1转录本分别增加了2.7倍和2.9倍。在所有研究的正常受试者中，PLAC1转录本表达完全为阴性。与正常样本相比，ALL中PLAC1 mRNA表达水平在统计学上显著升高（p=0.038）。然而，与正常受试者相比，AML中PLAC1的相对mRNA表达无统计学意义（p=0.848）。此外，AML各亚型中PLAC1的相对mRNA表达无统计学意义（p=0.756）。PLAC1基因表达在人口统计学、临床和准临床参数方面无差异。蛋白质印迹法证实了ALL和AML样本中PLAC1的表达。