Gossard F J, Chang A C, Cohen S N
Biochim Biophys Acta. 1986 Feb 24;866(1):68-74. doi: 10.1016/0167-4781(86)90102-8.
Messenger RNA was extracted from porcine pituitary pars intermedia and cloned as cDNA by standard methods. The nucleotide sequence encoding porcine pro-opiomelanocortin was established from analysis of two separate cDNA segments having an overlap of 420 bases. The amino acid sequence for the porcine pro-opiomelanocortin protein, which was inferred from the cDNA sequence, was found to correspond exactly to the sequence determined by direct amino acid analysis of the component peptides of pro-opiomelanocortin; namely, the porcine hormones ACTH, beta-lipotropin, gamma-MSH and the connecting peptide. We thus find no evidence for the existence of two porcine pro-opiomelanocortin genes that differ in their coding sequences, as was suggested by the in vitro protein synthesis results of others using mRNA obtained from porcine pituitaries.
从猪垂体中间部提取信使核糖核酸，并通过标准方法将其克隆为互补脱氧核糖核酸。通过对两个重叠420个碱基的独立互补脱氧核糖核酸片段进行分析，确定了编码猪阿黑皮素原的核苷酸序列。从互补脱氧核糖核酸序列推断出的猪阿黑皮素原蛋白的氨基酸序列，被发现与通过对阿黑皮素原组成肽进行直接氨基酸分析所确定的序列完全一致；即猪激素促肾上腺皮质激素、β-促脂素、γ-促黑素和连接肽。因此，我们没有发现证据表明存在两个编码序列不同的猪阿黑皮素原基因，而其他人使用从猪垂体获得的信使核糖核酸进行的体外蛋白质合成结果曾暗示存在这种情况。
