Barros Mirela Cesar, Pedrinha Victor Feliz, Graeff Marcia Sirlene Zardin, Bramante Clovis Monteiro, Duarte Marco Antonio Hungaro, de Andrade Flaviana Bombarda
Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
Integrated Research Center, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
Heliyon. 2023 Jul 6;9(7):e18042. doi: 10.1016/j.heliyon.2023.e18042. eCollection 2023 Jul.
To test and validate a new protocol for contamination of dentinal tubules using () by confocal laser scanning microscopy (CLSM), in addition to evaluating the effectiveness of conventional endodontic irrigants such as sodium hypochlorite (NaOCl) and chlorhexidine (CLX) on this biofilm.
Thirty lower premolars were contaminated with (ATCC 51190) for 7 days under anaerobic conditions using the proposed new model The specimens were divided into a control group and experimental groups, according to the irrigants: NaOCl 2.5% and CLX 2%. Then, the samples were submitted for analysis by CLSM and the LIVE/DEAD technique to quantify bacterial viability. Data normality was verified by the Shapiro-Wilk test. Intragroup and intergroup comparisons were performed using the Kruskal-Wallis test, followed by Dunn's post-test.
The CLSM images obtained demonstrated the effectiveness of the proposed new contamination protocol, with a high percentage of viable bacteria in relation to the treated groups (p < 0.05). Lower viability values were observed for the 2.5% NaOCl group.
The new contamination protocol resulted in a high and homogeneous percentage of viable bacteria in the dentinal tubules in all specimens evaluated. Both irrigating solutions proved to be effective in reducing the intratubular microbiota, especially 2.5% NaOCl.
使用（）通过共聚焦激光扫描显微镜（CLSM）测试并验证一种新的牙本质小管污染方案，此外评估传统根管冲洗剂如次氯酸钠（NaOCl）和氯己定（CLX）对这种生物膜的有效性。
使用所提出的新模型，在厌氧条件下用（ATCC 51190）对30颗下颌前磨牙进行污染7天。根据冲洗剂将标本分为对照组和实验组：2.5% NaOCl和2% CLX。然后，将样本提交进行CLSM分析和LIVE/DEAD技术以量化细菌活力。通过Shapiro-Wilk检验验证数据正态性。使用Kruskal-Wallis检验进行组内和组间比较，随后进行Dunn事后检验。
获得的CLSM图像证明了所提出的新污染方案的有效性，与处理组相比，存活细菌的百分比很高（p < 0.05）。2.5% NaOCl组观察到较低的活力值。
新的污染方案在所有评估标本的牙本质小管中产生了高且均匀的存活细菌百分比。两种冲洗液都被证明在减少管内微生物群方面有效，尤其是2.5% NaOCl。
