Kumburu Happiness H, Shayo Mariana, van Zwetslaar Marco, Njau Judith, Kuchaka Davis J, Ignas Ignas P, Wadugu Boaz, Kasworm Robert, Masaki Lazaro J, Hallgren Malte B, Clausen Philip T L C, Mmbaga Blandina Theophil, Aarestrup Frank M, Sonda Tolbert B
Kilimanjaro Christian Medical Centre (KCMC), Tanzania.
Kilimanjaro Clinical Research Institute (KCRI), Sokoine Road, P. O. Box. 2236, Moshi, Tanzania.
Heliyon. 2023 Jun 28;9(7):e17439. doi: 10.1016/j.heliyon.2023.e17439. eCollection 2023 Jul.
In resource-limited settings, patients are often first presented to clinical settings when seriously ill and access to proper clinical microbial diagnostics is often very limited or non-existing. On February 16th 2022 we were on a field trip to test a completely field-deployable metagenomics sequencing set-up, that includes DNA purification, sequencing, and bioinformatics analyses using bioinformatics tools installed on a laptop for water samples, just outside Moshi, Tanzania. On our way to the test site, we were contacted by the nearby Machame hospital regarding a child seriously ill with diarrhea and not responding to treatment. Within the same day, we conducted an onsite metagenomics examination of a fecal sample from the child, and was identified as the causative agent. The treatment was subsequently changed, with almost immediate improvement, and the child was discharged on February 21st.
在资源有限的环境中，患者往往在病情严重时才首次前往临床机构就诊，而获得适当的临床微生物诊断的机会通常非常有限或根本不存在。2022年2月16日，我们正在进行一次实地考察，以测试一套完全可在野外部署的宏基因组测序装置，该装置包括DNA纯化、测序以及使用安装在笔记本电脑上的生物信息学工具对水样进行生物信息学分析，地点就在坦桑尼亚莫希郊外。在我们前往测试地点的途中，附近的马查梅医院联系了我们，告知有一名患严重腹泻且治疗无反应的儿童。在同一天，我们对该儿童的粪便样本进行了现场宏基因组学检查，确定了病原体。随后更改了治疗方案，患儿几乎立即好转，并于2月21日出院。
