Cailloce Justine, Husson Fanny, Zablocki Aniela, Galy Vincent, Merlet Jorge
Sorbonne Université, CNRS, Institut de Biologie Paris Seine (IBPS), Developmental Biology Laboratory, UMR 7622, Paris, France.
MethodsX. 2023 Jul 20;11:102293. doi: 10.1016/j.mex.2023.102293. eCollection 2023 Dec.
is a model system widely used in fundamental research. Even though, nematodes are easy to maintain in the laboratory, obtaining large populations of worms require a lot of work and is time consuming. Furthermore, because are mainly hermaphrodite it is even more complicated to obtain large amounts of males which make high-throughput experiments using males very challenging. In order to overcome these limitations, we developed affordable and rapid methods to: (1) grow large synchronous worm populations (2) easily obtain large amounts of males We developed a culture method on plates to grow big synchronized worm populations with the standard incubators used on all worm labs. We also established an easy filtration method allowing to obtain large male populations in an hour. After filtering, the worm population contains more than 90% of adult males and no adult hermaphrodites since all the contaminants are larva and embryos. The culture and the filtering methods we developed are easy to implement and require a very limited investment in equipment and consumables beside the standard one present in worm labs. In addition, this filtering method could be applied to nematode's species similar in size to .
是一个在基础研究中广泛使用的模型系统。尽管线虫在实验室中易于饲养，但获得大量线虫需要大量工作且耗时。此外，由于线虫主要是雌雄同体，获得大量雄性线虫更加复杂，这使得使用雄性线虫进行高通量实验极具挑战性。为了克服这些限制，我们开发了经济且快速的方法来：（1）培养大量同步化的线虫群体（2）轻松获得大量雄性线虫。我们开发了一种平板培养方法，利用所有线虫实验室都使用的标准培养箱来培养大型同步化的线虫群体。我们还建立了一种简单的过滤方法，能够在一小时内获得大量雄性线虫。过滤后，线虫群体中成年雄性线虫的比例超过90%，且没有成年雌雄同体线虫，因为所有污染物都是幼虫和胚胎。我们开发的培养和过滤方法易于实施，除了线虫实验室现有的标准设备外，在设备和耗材方面所需投资非常有限。此外，这种过滤方法可应用于与线虫大小相似的线虫物种。
