Department of Animal Science and Technology, National Taiwan University, Taipei City, Taiwan.
Animal Technology Research Center, Agricultural Technology Research Institute, Miaoli County, Taiwan.
J Anim Physiol Anim Nutr (Berl). 2023 Nov;107(6):1368-1375. doi: 10.1111/jpn.13867. Epub 2023 Aug 4.
Weaning is a critical period in raising pigs. Novel animal feed additives that promote gut health and regulate immune function of piglets without antibiotics are needed. In this study, we aimed to test the ability of mesobiliverdin IXα-enriched microalgae (MBV IXα-enriched microalgae) to eliminate reliance on antibiotics to promote intestinal health in piglets. Eighty 28-day-old weaned piglets were randomly allocated to four groups each with four replicate pens and five piglets per pen. The dietary treatments were a basal diet as control (NC), basal diet plus 0.05% tylosin (PC), basal diet plus 0.1% or 0.5% MBV IXα-enriched microalgae as low (MBV-SP1) or high (MBV-SP2) dose respectively. All treated animals showed no significant differences in live weight, average daily gain and feed efficiency compared to control animals. Histological examination showed that MBV-SP1 and particularly MBV-SP2 increased the ratio of villus height to crypt depth in the jejunum and ileum compared to NC (p < 0.05). Similarly, tylosin treatment also increased villi lengths and the ratio of villus height to crypt depth in the jejunum and ileum compared to the NC (p < 0.05). MBV-SP1 and particularly MBV-SP2 reduced the levels of inflammatory cytokines interleukin-6 and tumour necrosis factor-alpha in the small intestine. MBV-SP2 and tylosin similarly reduced the lipid peroxidation marker (TBARS value) in the duodenum and ileum. In conclusion, feed supplementation with MBV IXα-enriched microalgae improved gut health by villus height and production of immunomodulators that correlated with down-regulated secretion of inflammatory cytokines.
断奶期是猪饲养的关键阶段。需要新型动物饲料添加剂来促进仔猪肠道健康并调节免疫功能，同时不依赖抗生素。在本研究中，我们旨在测试富含间胆绿素 IXα 的微藻（MBV IXα- 富集微藻）消除对仔猪抗生素的依赖以促进肠道健康的能力。将 80 头 28 日龄断奶仔猪随机分配到 4 个组，每个组有 4 个重复栏，每个栏有 5 头仔猪。日粮处理为基础日粮作为对照（NC），基础日粮加 0.05%酒石酸泰乐菌素（PC），基础日粮加 0.1%或 0.5% MBV IXα- 富集微藻作为低（MBV-SP1）或高（MBV-SP2）剂量。与对照动物相比，所有处理动物的活体重、平均日增重和饲料效率均无显著差异。组织学检查显示，MBV-SP1 特别是 MBV-SP2 增加了空肠和回肠中绒毛高度与隐窝深度的比值（p < 0.05）。同样，酒石酸泰乐菌素处理也增加了空肠和回肠中绒毛长度和绒毛高度与隐窝深度的比值（p < 0.05）。MBV-SP1 特别是 MBV-SP2 降低了小肠中炎症细胞因子白细胞介素-6 和肿瘤坏死因子-α的水平。MBV-SP2 和酒石酸泰乐菌素同样降低了十二指肠和回肠中脂质过氧化标志物（TBARS 值）的水平。总之，MBV IXα- 富集微藻的饲料补充改善了肠道健康，增加了绒毛高度和免疫调节剂的产生，这与炎症细胞因子分泌下调相关。
