通过气相色谱-负离子化学电离质谱法灵敏测定酪氨酸代谢产物对羟基苯乙酸、4-羟基-3-甲氧基苯乙酸和4-羟基-3-甲氧基扁桃酸。在一项稳定同位素标记示踪实验中的应用，以研究它们在人体内的代谢。
Sensitive determination of tyrosine metabolites, p-hydroxyphenylacetic acid, 4-hydroxy-3-methoxyphenyl-acetic acid and 4-hydroxy-3-methoxymandelic acid, by gas chromatography-negative-ion chemical-ionization mass spectrometry. Application to a stable isotope-labelled tracer experiment to investigate their metabolism in man.
作者信息
Shimamura M, Kamada S, Hayashi T, Naruse H, Iida Y
出版信息
J Chromatogr. 1986 Jan 10;374(1):17-26.
A method has been established for studying the dynamic metabolism of tyrosine to its metabolites in humans using a deuterium-labelled amino acid. Phenylalanine-d5 was administered orally to human subjects (5 mg/kg) and the levels of p-hydroxyphenylacetic acid-d4, 4-hydroxy-3-methoxyphenylacetic acid-d3, and 4-hydroxy-3-methoxymandelic acid-d3 excreted into urine every hour were determined by gas chromatography-negative-ion chemical-ionization mass spectrometry. This method was also applied to some patients with depression and it was possible to detect a slight alteration in the excretion of some compounds compared with the control.
已经建立了一种使用氘标记氨基酸研究人体中酪氨酸向其代谢产物的动态代谢的方法。向人体受试者口服苯丙氨酸-d5（5mg/kg），并通过气相色谱-负离子化学电离质谱法测定每小时排泄到尿液中的对羟基苯乙酸-d4、4-羟基-3-甲氧基苯乙酸-d3和4-羟基-3-甲氧基扁桃酸-d3的水平。该方法也应用于一些抑郁症患者，与对照组相比，可以检测到某些化合物排泄的轻微变化。
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