Jacob K, Vogt W, Schwertfeger G
J Chromatogr. 1984 May 4;290:331-7. doi: 10.1016/s0021-9673(01)93587-3.
A sensitive and simple method for the simultaneous quantitation of homovanillic acid and vanilmandelic acid in small samples (0.1-1.0 ml) of urine, serum, plasma and cerebrospinal fluid is described. The stable dimethylthiophosphinyl methyl ester derivatives are detected specifically by mass fragmentography using the respective deuterated compounds as internal standards. Gas chromatographic separation is performed on a fused-silica DB-1 capillary column combined with a cold injection system for large sample volumes. Linear response curves and a detection limit of 1 ng/ml are obtained. The method has been applied to the localization of pheochromocytoma by selective determination of venous vanilmandelic acid.
本文描述了一种灵敏且简便的方法，用于同时定量测定尿液、血清、血浆和脑脊液的小样本（0.1 - 1.0毫升）中的高香草酸和香草扁桃酸。使用各自的氘代化合物作为内标，通过质量碎片分析法特异性检测稳定的二甲基硫代磷酰甲酯衍生物。在与大体积样品冷进样系统联用的熔融石英DB - 1毛细管柱上进行气相色谱分离。获得了线性响应曲线，检测限为1纳克/毫升。该方法已应用于通过选择性测定静脉血香草扁桃酸来定位嗜铬细胞瘤。
