Synergistic effect of hydralazine associated with triazoles on spp. in planktonic cells.

To evaluate the antifungal activity of hydralazine hydrochloride alone and in synergy with azoles against spp. and the action mechanism. We used broth microdilution assays to determine the MIC, checkerboard assays to investigate synergism, and flow cytometry and molecular docking tests to ascertain action mechanism. Hydralazine alone had antifungal activity in the range of 16-128 μg/ml and synergistic effect with itraconazole versus 100% of the fungal isolates, while there was synergy with fluconazole against 11.11% of the isolates. There was molecular interaction with the receptors exo-B(1,3)-glucanase and CYP51, causing reduced cell viability and DNA damage. Hydralazine is synergistic with itraconazole and triggers cell death of spp. at low concentrations, demonstrating antifungal potential.