Holanda Maria Av, da Silva Cecília R, de A Neto João B, do Av Sá Lívia G, do Nascimento Francisca Bsa, Barroso Daiana D, da Silva Lisandra J, Cândido Thiago M, Leitão Amanda C, Barbosa Amanda D, de Moraes Manoel O, Cc Bruno, Júnior Hélio V Nobre
Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE 60430 1160, Brazil.
Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE 60430 276, Brazil.
Future Microbiol. 2021 Jan;16(2):71-81. doi: 10.2217/fmb-2020-0080. Epub 2021 Jan 18.
The purpose of this study was to evaluate the antifungal activity of midazolam, alone and in association with azoles, against isolates of clinical spp. in planktonic and biofilm form. The antifungal activity was observed using the broth microdilution technique. Flow cytometry tests were performed to investigate the probable mechanism of action and the comet test and cytotoxicity test were applied to evaluate DNA damage. Midazolam (MIDAZ) showed antifungal activity against planktonic cells (125-250 μg/ml) and reduced the viability of spp. biofilms (125 a 2500 μg/ml). The interaction of MIDAZ against spp. biofilms was observed through scanning electron microscopy, causing alteration of their appearance. Therefore, MIDAZ has antifungal potential against spp.
本研究的目的是评估咪达唑仑单独及与唑类联合使用时，对浮游和生物膜形式的临床分离菌株的抗真菌活性。采用肉汤微量稀释技术观察抗真菌活性。进行流式细胞术试验以研究可能的作用机制，并应用彗星试验和细胞毒性试验评估DNA损伤。咪达唑仑（MIDAZ）对浮游细胞显示出抗真菌活性（125 - 250μg/ml），并降低了生物膜的活力（125至2500μg/ml）。通过扫描电子显微镜观察到MIDAZ与生物膜的相互作用，导致其外观改变。因此，MIDAZ对具有抗真菌潜力。
