Li Y, Turck C M, Teumer J K, Stavnezer E
J Virol. 1986 Mar;57(3):1065-72. doi: 10.1128/JVI.57.3.1065-1072.1986.
The Sloan-Kettering viruses (SKVs) are a group of transforming retroviruses that were isolated from chicken embryo cells which had been infected with the avian leukosis virus transformation-defective Bratislava 77 (tdB77). Each of the SKV isolates was shown to contain multiple genomes of different sizes indicating the presence of several viruses in addition to tdB77. To identify and characterize the putative transforming gene(s) of the SKVs, we used hybridization selection to isolate the fraction of a representative cDNA which was SKV specific. Both solution and blot hybridization studies with viral RNAs showed that the specific probe contained a sequence, ski, that was at least partially held in common by the multiple SKV genomes. This conclusion was confirmed by the observation that a molecularly cloned ski probe also hybridized to each of the multiple SKV genomes. Southern blots of chicken DNA revealed homologs of ski (c-ski) which were not associated with endogenous viral loci. Results showing that c-ski was expressed in polyadenylated cytoplasmic RNA of uninfected chicken cells indicated that it is a functional gene. Other data showed that c-ski was conserved in avian and mammalian evolution, suggesting a functional role for the gene in species other than chickens. Using ski cDNA in solution hybridizations with viral RNAs and in Southern blot hybridization with cloned retroviral oncogenes, we did not detect any relationship between ski and any of 15 previously identified oncogenes.
斯隆-凯特琳病毒（SKVs）是一组转化逆转录病毒，它们是从感染了禽白血病病毒转化缺陷型布拉迪斯拉发77（tdB77）的鸡胚细胞中分离出来的。每个SKV分离株都显示含有多个不同大小的基因组，这表明除了tdB77之外还存在几种病毒。为了鉴定和表征SKVs的假定转化基因，我们使用杂交筛选来分离具有SKV特异性的代表性cDNA片段。对病毒RNA进行的溶液杂交和印迹杂交研究均表明，特异性探针包含一个ski序列，该序列至少部分为多个SKV基因组所共有。分子克隆的ski探针也能与多个SKV基因组中的每一个杂交，这一观察结果证实了这一结论。鸡DNA的Southern印迹显示了ski（c-ski）的同源物，它们与内源性病毒位点无关。结果表明c-ski在未感染鸡细胞的多聚腺苷酸化细胞质RNA中表达，这表明它是一个功能基因。其他数据表明c-ski在鸟类和哺乳动物进化过程中是保守的，这表明该基因在鸡以外的物种中也具有功能作用。使用ski cDNA与病毒RNA进行溶液杂交，并与克隆的逆转录病毒癌基因进行Southern印迹杂交，我们未检测到ski与15个先前鉴定的癌基因中的任何一个之间存在任何关系。
