Zhang Jingxiao, Sun Jian, Zhang Yu, Zhang Min, Liu Xin, Yang Longfei, Yin Yongjie
Department of Emergency and Critical Care Medicine, The Second Hospital of Jilin University, 218# Ziqiang Street, Changchun, 130041, China.
Department of Clinical Laboratory, The Second Hospital of Jilin University, 218# Ziqiang Street, Changchun, 130041, China.
AMB Express. 2023 Aug 4;13(1):82. doi: 10.1186/s13568-023-01587-y.
Candida albicans infections are threatening public health but there are only several antifungal drugs available. This study was to assess the effects of dehydrocostus lactone (DL) on the Candida albicans growth and biofilms Microdilution assays revealed that DL inhibits a panel of standard Candida species, including C. albicans, as well as 9 C. albicans clinical isolates. The morphological transition of C. albicans in RPMI-1640 medium and the adhesion to polystyrene surfaces can also be decreased by DL treatment, as evidenced by microscopic, metabolic activity and colony forming unit (CFU) counting assays. The XTT assay and microscopy inspection demonstrated that DL can inhibit the biofilms of C. albicans. Confocal microscopy following propidium iodide (PI) staining and DCFH-DA staining after DL treatment revealed that DL can increase the membrane permeability and intracellular reactive oxygen species (ROS) production. N-acetyl-cysteine could mitigate the inhibitory effects of DL on growth, morphological transition and biofilm formation, further confirming that ROS production induced by DL contributes to its antifungal and antibiofilm effects. This study showed that DL demonstrated antifungal and antibiofilm activity against C. albicans. The antifungal mechanisms may involve membrane damage and ROS overproduction. This study shows the potential of DL to fight Candida infections.
白色念珠菌感染正威胁着公众健康，但目前可用的抗真菌药物仅有几种。本研究旨在评估去氢木香内酯（DL）对白色念珠菌生长和生物膜的影响。微量稀释试验表明，DL对包括白色念珠菌在内的一组标准念珠菌物种以及9株白色念珠菌临床分离株均有抑制作用。通过显微镜检查、代谢活性和菌落形成单位（CFU）计数试验证明，DL处理还可降低白色念珠菌在RPMI-1640培养基中的形态转变以及对聚苯乙烯表面的黏附。XTT试验和显微镜检查表明，DL可抑制白色念珠菌生物膜的形成。DL处理后进行碘化丙啶（PI）染色和2',7'-二氯二氢荧光素二乙酸酯（DCFH-DA）染色后的共聚焦显微镜检查显示，DL可增加细胞膜通透性并促进细胞内活性氧（ROS）的产生。N-乙酰半胱氨酸可减轻DL对生长、形态转变和生物膜形成的抑制作用，进一步证实DL诱导产生的ROS有助于其抗真菌和抗生物膜作用。本研究表明，DL对白色念珠菌具有抗真菌和抗生物膜活性。其抗真菌机制可能涉及膜损伤和ROS过量产生。本研究显示了DL对抗念珠菌感染的潜力。
