Fathi-Hafshejani Parvin, Tinker Hunter B, Freel Katherine, Mahjouri-Samani Masoud, Hasim Sahar
Department of Electrical and Computer Engineering, Auburn University, Auburn, Alabama36849, United States.
Department of Biology, Mercer University, Macon, Georgia31207, United States.
ACS Appl Bio Mater. 2023 Feb 20;6(2):436-444. doi: 10.1021/acsabm.2c00707. Epub 2023 Feb 1.
Titanium is widely used in medical devices, such as dental and orthopedic implants, due to its excellent mechanical properties, low toxicity, and biocompatibility. However, the titanium surface has the risk of microbial biofilm formation, which results in infections from species such as (). This kind of biofilm prevents antifungal therapy and complicates the treatment of infectious diseases associated with implanted devices. It is critical to developing a feasible surface to decrease microbial growth while not interfering with the growth of the host cells. This study reports the influence of titanium surface modification to titanium disulfide (TiS) on inhibiting biofilm formation while allowing the attachment of human gingival fibroblasts (HGFs) on their surface. The surface of titanium parts is directly converted to structured titanium and TiS using direct laser processing and crystal growth methods. adhesion and colonization are then investigated on these surfaces by the colony counting assay and reactive oxygen species (ROS) assay, using 2',7'-dichlorofluorescin diacetate (DCFH-DA) and microscopy images. Also, the viability and adhesion of HGFs on these surfaces are investigated to show their adhesion and biocompatibility. Titanium samples with the TiS surface show both biofilm inhibition and HGF attachment. This study provides insight into designing and manufacturing titanium biomedical implants.
钛因其优异的机械性能、低毒性和生物相容性而广泛应用于医疗设备，如牙科和骨科植入物。然而，钛表面存在微生物生物膜形成的风险，这会导致诸如（）等物种的感染。这种生物膜会妨碍抗真菌治疗，并使与植入设备相关的传染病治疗复杂化。开发一种可行的表面以减少微生物生长同时不干扰宿主细胞的生长至关重要。本研究报告了钛表面改性为二硫化钛（TiS）对抑制生物膜形成的影响，同时允许人牙龈成纤维细胞（HGFs）附着在其表面。使用直接激光加工和晶体生长方法将钛部件的表面直接转化为结构化钛和TiS。然后通过菌落计数法和活性氧（ROS）测定法，使用2',7'-二氯荧光素二乙酸酯（DCFH-DA）和显微镜图像，研究这些表面上的（）粘附和定植情况。此外，还研究了HGFs在这些表面上的活力和粘附情况，以显示它们的粘附性和生物相容性。具有TiS表面的钛样品既显示出对生物膜的抑制作用，又显示出HGFs的附着。本研究为设计和制造钛生物医学植入物提供了见解。
