Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Saveh University of Medical Sciences, Saveh, Iran.
Mol Biol Rep. 2023 Oct;50(10):8073-8079. doi: 10.1007/s11033-023-08704-x. Epub 2023 Aug 4.
Uropathogenic Escherichia coli (UPEC) is a major pathogen of the urinary tract infection (UTI), and biofilm formation is crucial as it facilitates the colonization in the urinary tract. We aimed to investigate the antibiotic susceptibility pattern, biofilm formation capability, distribution of quinolone resistance genes, and phylogenetic groups among UPEC isolates from an Iranian inpatients' community.
A collection of 126 UPEC obtained from hospitalized patients with symptomatic UTI at 3 teaching hospitals during 2016 were included. Antibiogram of all isolates against quinolone and fluoroquinolones was performed using the disk diffusion method. Phylogenetic groups and qnr A, B, and S genes were assessed by PCR. Susceptibility pattern showed that more than 50% and 81% of the isolates were resistant to fluoroquinolones and quinolones, correspondingly. The frequency of qnrS and qnrB genes was 22% and 13.5%, correspondingly. Our result indicated no significant association between the presence of fluoroquinolone genes and antibiotic resistance to them. The frequent common phylogroup was B2 (84.1%), followed by D (10.3%), A (3.2%) and B1 (2.4%) groups. Indeed, 80.2% of the isolates were biofilm producers, so that 42.1%, 16.7% and 21.4% of them were classified as weak, moderate and strong producers, respectively.
Our results showed considerable fluoroquinolone and quinolone resistance among UPEC along with a remarkable rate of biofilm-producing isolates from symptomatic hospitalized patients, making them a serious health concern in the region. This survey highlights the need for awareness on quinolone resistance and careful prescription of them by physicians.
尿路致病性大肠杆菌（UPEC）是尿路感染（UTI）的主要病原体，其生物膜形成能力至关重要，因为它有助于在泌尿道中定植。我们旨在研究来自伊朗住院患者社区的 UPEC 分离株的抗生素敏感性模式、生物膜形成能力、喹诺酮耐药基因的分布和系统发育群。
本研究纳入了 2016 年 3 家教学医院住院的有症状 UTI 患者中获得的 126 株 UPEC。采用纸片扩散法检测所有分离株对喹诺酮类和氟喹诺酮类的抗生素谱。通过 PCR 评估系统发育群和 qnrA、B 和 S 基因。药敏试验结果显示，超过 50%和 81%的分离株分别对氟喹诺酮类和喹诺酮类耐药。qnrS 和 qnrB 基因的频率分别为 22%和 13.5%。我们的结果表明，氟喹诺酮类基因的存在与对它们的抗生素耐药性之间没有显著关联。常见的共同 phylogroup 是 B2（84.1%），其次是 D（10.3%）、A（3.2%）和 B1（2.4%）。事实上，80.2%的分离株是生物膜生产者，其中 42.1%、16.7%和 21.4%分别为弱、中和强生产者。
我们的研究结果显示，来自有症状住院患者的 UPEC 中存在相当高的氟喹诺酮类和喹诺酮类耐药率，以及相当高的生物膜产生分离株比例，这使得它们成为该地区的一个严重健康问题。本研究强调了对喹诺酮类耐药性的认识和医生谨慎处方此类药物的必要性。
