Palutke M, Schnitzer B, Narang S, Hyder D M, Parks S, Tabaczka P, Patch J
Am J Clin Pathol. 1986 Apr;85(4):494-7. doi: 10.1093/ajcp/85.4.494.
Immunoperoxidase staining of frozen sections is a cost-effective technic for immunophenotyping cells of lymphoid tissue. Because this procedure is not performed in many institutions, a simple method to transport fresh tissue to centers performing these studies is required. Tissues in saline at refrigerator temperature may be successfully transported. In addition, in order to minimize laboratory expenses, lymphoid tissue can be kept refrigerated in saline until permanent sections are examined and immunodiagnostic procedures become necessary. In this study reproducible immunophenotyping of 12 samples of lymphoid tissue stored up to seven days was achieved.
冷冻切片的免疫过氧化物酶染色是对淋巴组织细胞进行免疫表型分析的一种经济有效的技术。由于许多机构不开展此操作，因此需要一种将新鲜组织运送至进行这些研究的中心的简单方法。处于冰箱温度盐水中的组织可成功运送。此外，为了尽量减少实验室费用，淋巴组织可保存在盐水中冷藏，直至检查永久切片并需要进行免疫诊断程序。在本研究中，对保存长达7天的12份淋巴组织样本进行了可重复的免疫表型分析。
