Chatelet F, Brianti E, Ronco P, Roland J, Verroust P
Am J Pathol. 1986 Mar;122(3):512-9.
An immunoultrastructural study has been undertaken for analysis of the extrarenal localization of two brush border (BB) glycoproteins of 90 kd and 330 kd expressed by glomeruli. In previous studies on renal tissue, it has been shown that gp 330 is restricted to coated pits and BB intermicrovillar invaginations, whereas gp 90 is diffusely distributed on epithelial cell membranes and in addition expressed on glomerular endothelial cells. At variance from observations made in the kidney, gp 330 and gp 90 are mainly expressed on different epithelial cell types: gp 330 is found on the coated pits of the epithelium lining the epididymis and on the entire surface of the microvilli of Type II pneumocytes, whereas gp 90 is detected on the gut brush border and the biliary pole of hepatocytes; gp 90 is also found on endothelia lining all distal capillary beds studied in heart, lung, liver, and spleen. These observations thus indicate that gp 90 is expressed by a wide variety of cell types, all associated with high membrane turnover. Conversely, gp 330 has a restricted distribution at the organ, cellular and subcellular level and may be related with a defined system of receptor-mediated endocytosis.
已开展一项免疫超微结构研究，以分析肾小球表达的两种90kd和330kd刷状缘（BB）糖蛋白的肾外定位。在先前对肾组织的研究中，已表明gp 330局限于被覆小窝和BB微绒毛间内陷，而gp 90则弥散分布于上皮细胞膜上，此外还表达于肾小球内皮细胞。与在肾脏中的观察结果不同，gp 330和gp 90主要表达于不同的上皮细胞类型：gp 330见于附睾衬里上皮的被覆小窝以及II型肺泡上皮细胞微绒毛的整个表面，而gp 90则在肠刷状缘和肝细胞的胆小管极检测到；在心脏、肺、肝脏和脾脏中研究的所有远端毛细血管床的内皮细胞上也发现了gp 90。因此，这些观察结果表明gp 90由多种细胞类型表达，所有这些细胞类型都与高膜更新有关。相反，gp 330在器官、细胞和亚细胞水平上分布受限，可能与特定的受体介导的内吞系统有关。
