Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Talanta. 2024 Jan 1;266(Pt 1):125014. doi: 10.1016/j.talanta.2023.125014. Epub 2023 Jul 31.
Rolling circle amplification (RCA) process as an excellent DNA amplifier strategy possesses the merits of high performance and easy operation. In this research, a sensitive RCA-based fluorescent aptasensor was fabricated for the detection of kanamycin residues in food. The aptasensing approach consisted of two main steps; immobilization of biotinylated kanamycin aptamer on streptavidin magnetic beads (SMB) and separation of free complementary strands (CS) from the SMB-aptamer/kanamycin at the first step. For the second step, RCA procedure was applied as signal magnifier and SYBR Green was added as fluorescent indicator dye. The linear relation between the aptasensor response and kanamycin concentration was obtained from 5 nM to 100 nM with the detection limit of 1.93 nM (S/N = 3). The aptasensor displayed satisfactory selectivity among other antibiotics. The developed aptasensor is reliable for monitoring kanamycin in milk as a common foodstuff.
滚环扩增（RCA）作为一种出色的 DNA 扩增策略，具有高性能和易操作的优点。本研究构建了一种基于 RCA 的灵敏荧光适体传感器，用于检测食品中的卡那霉素残留。适体传感方法包括两个主要步骤：首先，将生物素化的卡那霉素适体固定在链霉亲和素磁珠（SMB）上，并将游离的互补链（CS）从 SMB-适体/卡那霉素中分离出来。第二步，应用 RCA 程序作为信号放大器，并添加 SYBR Green 作为荧光指示剂染料。该适体传感器在 5 nM 至 100 nM 的范围内表现出与卡那霉素浓度的线性关系，检测限为 1.93 nM（S/N = 3）。该适体传感器在其他抗生素中表现出良好的选择性。该开发的适体传感器可用于监测牛奶等常见食品中的卡那霉素。
