Department of Biochemistry, CSIR-Central Food Technological Research Institute (CFTRI), Mysuru, 570020, Karnataka, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India.
Central Instrumentation Facility and Service, CSIR-Central Food Technological Research Institute (CFTRI), Mysuru, 570020, Karnataka, India.
Carbohydr Res. 2023 Oct;532:108914. doi: 10.1016/j.carres.2023.108914. Epub 2023 Jul 28.
Garden cress seeds produces mucilage that has found various food applications, however, there is little information on the free oligosaccharides (FOS) contents in these seeds. Herein, we explored the presence of FOS in cress seed aqueous exudate. PGC-LC MS/MS analysis indicated the presence of mainly hexose containing oligosaccharides such as raffinose, stachyose and verbascose belonging to raffinose family of oligosaccharides (RFOs). In addition, minor fraction of planteose, isomeric tri- and tetrasaccharides were also observed. Further, the structural confirmation of the abundant tri- and tetrasaccharide were obtained through 1D and 2D NMR analysis. Thus, the RFOs presence in cress seeds would enhance its bio-functionalities.
荠菜籽产生的黏液在各种食品应用中都有发现，然而，关于这些种子中游离低聚糖（FOS）含量的信息却很少。在此，我们研究了 FOS 在荠菜籽水渗出物中的存在。PGC-LC MS/MS 分析表明，主要存在含有己糖的低聚糖，如属于棉子糖家族低聚糖（RFOs）的棉子糖、水苏糖和毛蕊花糖。此外，还观察到少量的车前糖、异构的三糖和四糖。此外，通过 1D 和 2D NMR 分析获得了丰富的三糖和四糖的结构确证。因此，荠菜籽中 RFOs 的存在将增强其生物功能。
