Aquatic and Crop Resource Development Research Centre, National Research Council of Canada, 6100 Royalmount Ave., Montreal, Quebec H4P 2R2, Canada.
Aquatic and Crop Resource Development Research Centre, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario K1A 0R6, Canada.
Bioresour Technol. 2023 Nov;387:129595. doi: 10.1016/j.biortech.2023.129595. Epub 2023 Aug 2.
Despite known metabolic versatility of Burkholderia spp., sugar metabolism and end-product synthesis patterns in Burkholderia thailandensis have been poorly characterized. This work has demonstrated that B. thailandensis is capable of simultaneously uptaking glucose and xylose and accumulating up to 64% of its dry mass as poly(3-hydroxyalkanoate) (PHA) biopolymers, resulting in a PHA titer of up to 3.8 g L in shake flasks. Rhamnolipids - mainly (68-75%) in the form of Rha-Rha-C-C - were produced concomitantly with a titer typically in the range of 0.2-0.4 g L. Gluconic and xylonic acids were also detected in titers of up to 5.3 g L, and while gluconic acid appeared to be back consumed, xylonic acid formed as a major end product. This first example of co-production of three products from mixed sugars using B. thailandensis paves the way for improving biorefinery economics.
尽管已知伯克霍尔德氏菌属具有已知的代谢多样性，但泰国伯克霍尔德氏菌的糖代谢和终产物合成模式仍未得到很好的描述。这项工作表明，泰国伯克霍尔德氏菌能够同时摄取葡萄糖和木糖，并将其干重的 64%累积为聚（3-羟基烷酸酯）（PHA）生物聚合物，导致摇瓶中的 PHA 滴度高达 3.8 g/L。鼠李糖脂 - 主要以 Rha-Rha-C-C 的形式存在 - 与通常在 0.2-0.4 g/L 范围内的滴度同时产生。也检测到葡萄糖酸和木酮糖酸的浓度高达 5.3 g/L，尽管葡萄糖酸似乎被回吸，但木酮糖酸是主要的终产物。这是首次使用泰国伯克霍尔德氏菌从混合糖中同时生产三种产品的例子，为提高生物精炼经济提供了途径。
