Hecht N B, Bower P A, Waters S H, Yelick P C, Distel R J
Exp Cell Res. 1986 May;164(1):183-90. doi: 10.1016/0014-4827(86)90465-9.
Hybridization of RNA blots of total testicular RNA from prepuberal and sexually mature CD1 mice with several mouse testicular cDNA probes reveals that the mRNA encoding the two mouse protamines, an actin sequence of 1.5 kb, and a post-meiotically expressed 620 nucleotide mRNA are first detected in the testes of mice 22 days of age. These experiments and other studies analysing RNA preparations from isolated populations of testicular cell types with cDNA probes [1, 2] demonstrate that haploid gene expression occurs in the mammalian testis.
用几种小鼠睾丸cDNA探针与青春期前和性成熟的CD1小鼠的睾丸总RNA印迹进行杂交，结果显示，编码两种小鼠鱼精蛋白的mRNA、一个1.5kb的肌动蛋白序列以及一个减数分裂后表达的620个核苷酸的mRNA，首次在22日龄小鼠的睾丸中被检测到。这些实验以及其他用cDNA探针分析从分离的睾丸细胞类型群体中制备的RNA的研究[1,2]表明，单倍体基因表达发生在哺乳动物睾丸中。
