Wright J R, Wager R E, Hamilton R L, Huang M, Clements J A
J Appl Physiol (1985). 1986 Mar;60(3):817-25. doi: 10.1152/jappl.1986.60.3.817.
The goals of this investigation were to determine whether subfractions of alveolar surfactant that have different physical and biochemical properties are preferentially taken up from the alveolar air space into lamellar bodies and to correlate the magnitude of the uptake with the properties of the fractions. Radiolabeled subfractions were obtained by differential centrifugation of lavage fluid from rabbits that had been intravenously injected with radioactive palmitate. The subfractions were P (pellet) 3 (1,000 g, 20 min), P4 (60,000 g, 60 min), P5 (100,000 g, 16 h). Subfractions were instilled into the lungs of anesthetized spontaneously breathing adult rabbits, and lavage and lamellar body fractions were isolated at later times. P3 and P4 were taken up to a larger extent than was P5 or liposomes prepared from a P4 lipid extract. The fractions that were preferentially taken up (P3 and P4) contained surfactant apoprotein (APO) 36, tubular myelin, multilamellar vesicles, and were rapidly adsorbed to an air-water interface. P3 also contained APO 10. These results demonstrate that different forms of surfactant are recycled at different rates and suggest that there is specificity in the recycling process.
本研究的目的是确定具有不同物理和生化特性的肺泡表面活性物质亚组分是否优先从肺泡气腔被摄取到板层小体中，并将摄取量与各亚组分的特性相关联。通过对静脉注射放射性棕榈酸的家兔灌洗液进行差速离心获得放射性标记的亚组分。亚组分分别为P（沉淀）3（1000g，20分钟）、P4（60000g，60分钟）、P5（100000g，16小时）。将亚组分滴注到麻醉状态下自主呼吸的成年家兔肺中，随后分离灌洗和板层小体组分。P3和P4的摄取程度比P5或由P4脂质提取物制备的脂质体更大。优先被摄取的组分（P3和P4）含有表面活性物质载脂蛋白（APO）36、管状髓鞘、多层囊泡，并且能迅速吸附到气-水界面。P3还含有APO 10。这些结果表明不同形式的表面活性物质以不同速率进行再循环，提示再循环过程存在特异性。
