Baritussio A, Bellina L, Carraro R, Rossi A, Enzi G, Magoon M W, Mussini I
Eur J Clin Invest. 1984 Feb;14(1):24-9. doi: 10.1111/j.1365-2362.1984.tb00699.x.
Rabbit alveolar saturated phosphatidylcholine (SPC) can be separated by differential and density gradient centrifugation of lung lavage into three fractions. One fraction ('B'), which is analogous to conventionally prepared alveolar surfactant, contains 46.0 +/- 5.9% (SD) of lavage SPC, and is made of large multilamellar vesicles or sheet-like structures. A second fraction ('C') does not sediment after centrifugation at 80 000 g for 90 min, contains 29.8 +/- 14.0% of lavage SPC, and a uniform population of small vesicles. This fraction incorporates 3H-palmitate administered intravenously with a small delay with respect to fraction 'B'. A third fraction ('D') contains almost all the cells of lavage and less than 5% of lavage SPC. We conclude that alveolar SPC consists of more than one compartment. The possible significance of isolated fractions is discussed.
兔肺泡饱和磷脂酰胆碱（SPC）可通过对肺灌洗物进行差速离心和密度梯度离心分离为三个组分。其中一个组分（“B”）类似于传统制备的肺泡表面活性物质，含有灌洗物中46.0±5.9%（标准差）的SPC，由大的多层囊泡或片状结构组成。第二个组分（“C”）在80000g离心90分钟后不沉淀，含有灌洗物中29.8±14.0%的SPC，以及均匀的小囊泡群体。该组分在静脉注射3H-棕榈酸酯后，相对于组分“B”有一小段延迟才摄取。第三个组分（“D”）几乎包含灌洗物中的所有细胞，以及少于5%的灌洗物SPC。我们得出结论，肺泡SPC由多个区室组成。文中讨论了分离组分可能具有的意义。
