Department of Food Science and Technology, Oregon State University, 3051 SW Campus Way, Corvallis, OR, 97331, USA.
Institute of Life Sciences & Resources and Department of Food Science and Technology, Kyung-Hee University, Gyeonggi-Do, Yongin, 17104, Republic of Korea.
Appl Microbiol Biotechnol. 2023 Oct;107(19):6047-6056. doi: 10.1007/s00253-023-12693-z. Epub 2023 Aug 5.
Listeria monocytogenes is a pathogenic bacterium which can live in adverse environments (low pH, high salinity, and low temperature). Even though there are various whole genome sequencing (WGS) data on L. monocytogenes, investigations on genetic differences between stress-resistant and -sensitive L. monocytogenes grown under stress environments have been not fully examined. This study aims to investigate and compare genetic characteristics between stress-resistant and -sensitive L. monocytogenes using whole genome sequencing (WGS). A total of 47 L. monocytogenes strains (43 stress-resistant and 4 stress-sensitive) were selected based on the stress-resistance tests under pH 3, 5% salt concentration, and 1 °C. The sequencing library for WGS was prepared and sequenced using an Illumina MiSeq. Genetic characteristics of two different L. monocytogenes groups were examined to analyze the pangenome, functionality, virulence, antibiotic resistance, core, and unique genes. The functionality of unique genes in the stress-resistant L. monocytogenes was distinct compared to the stress-sensitive L. monocytogenes, such as carbohydrate and nucleotide transport and metabolism. The lisR virulence gene was detected more in the stress-resistant L. monocytogenes than in the stress-sensitive group. Five stress-resistant L. monocytogenes strains possessed tet(M) antibiotic resistance gene. This is the first study suggesting that deep genomic characteristics of L. monocytogenes may have different resistance level under stress conditions. This new insight will aid in understanding the genetic relationship between stress-resistant and -sensitive L. monocytogenes strains isolated from diverse resources. KEY POINTS: • Whole genomes of L. monocytogenes isolated from three different sources were analyzed. • Differences in two L. monocytogenes groups were identified in functionality, virulence, and antibiotic resistance genes. • This study first examines the association between resistances and whole genomes of stress-resistant and -sensitive L. monocytogenes.
李斯特菌是一种能在恶劣环境（低 pH 值、高盐度和低温）中生存的致病菌。尽管有大量关于李斯特菌的全基因组测序（WGS）数据，但对在应激环境下生长的抗应激和敏感李斯特菌之间的遗传差异的研究尚未完全进行。本研究旨在通过全基因组测序（WGS）研究和比较抗应激和敏感李斯特菌的遗传特征。根据在 pH3、5%盐浓度和 1°C 下的应激抗性测试，共选择了 47 株李斯特菌（43 株抗应激和 4 株应激敏感）。为 WGS 制备测序文库并使用 Illumina MiSeq 进行测序。检查了两种不同李斯特菌群体的遗传特征，以分析泛基因组、功能、毒力、抗生素耐药性、核心和独特基因。与应激敏感李斯特菌相比，应激抗性李斯特菌独特基因的功能明显不同，如碳水化合物和核苷酸的运输和代谢。在应激抗性李斯特菌中检测到更多的 lisR 毒力基因，而在应激敏感组中则较少。五株应激抗性李斯特菌携带 tet(M)抗生素耐药基因。这是第一项表明李斯特菌深层基因组特征可能在应激条件下具有不同耐药水平的研究。这一新的见解将有助于理解从不同资源中分离出的抗应激和敏感李斯特菌菌株之间的遗传关系。 关键点： • 分析了从三个不同来源分离的李斯特菌的全基因组。 • 在功能、毒力和抗生素耐药基因方面，鉴定了两个李斯特菌群体之间的差异。 • 本研究首次研究了应激抗性和应激敏感李斯特菌的耐药性与全基因组之间的关系。
