School of Food and Health, Beijing Technology and Business University, Beijing, China; Institute of Food Safety, Science and Technology Research Center of China Customs, Beijing, China.
Institute of Food Safety, Science and Technology Research Center of China Customs, Beijing, China.
Food Res Int. 2022 Sep;159:111641. doi: 10.1016/j.foodres.2022.111641. Epub 2022 Jul 8.
Listeria monocytogenes is a food-borne pathogen that can cause human listeriosis. The main cause of L.monocytogenes poisoning is the consumption of contaminated or processed foods, of which meat is the main one. At present, it is not clear whether the listeria contaminated in different meatis genetically diverse. In this study, a total of 90 L.monocytogenes isolates including 28 isolates in livestock meat and 62 isolates in poultry meat were obtained from chicken, duck, pork, and beef respectively in Beijing. And whole-genome sequencing (WGS) based analysis was performed to investigate the genetic diversity of L.monocytogenes between poultry and livestock meat isolates. As a result, we found the prevalence of the multilocus sequence typing (MLST)-derived clonal complexes (CCs) were quite different between two different types of meat. The number of resistance genes and virulence genes in most of the L.monocytogenes isolated from the four meat species were close, but the sequences of eight resistance genes and 19 virulence genes were significantly varied between poultry meat and livestock meat. The phylogenetic and collinear analysis of these isolates revealed the isolates in the same meat have a similar genome. These results showed that the genetic variation of L.monocytogenes in livestock and poultry meat were significantly different isolates.
李斯特菌是一种食源性病原体，可以导致人类李斯特菌病。李斯特菌中毒的主要原因是食用受污染或加工的食物，其中肉类是主要来源。目前，尚不清楚不同肉类中污染的李斯特菌是否具有遗传多样性。在本研究中，我们从北京的鸡肉、鸭肉、猪肉和牛肉中分别获得了 90 株李斯特菌分离株，包括 28 株畜肉分离株和 62 株禽肉分离株。并进行了基于全基因组测序（WGS）的分析，以调查禽肉和畜肉分离株中李斯特菌的遗传多样性。结果发现，两种不同类型的肉中，基于多位点序列分型（MLST）的克隆群（CC）的流行率有很大差异。大多数从四种肉类中分离的李斯特菌的耐药基因和毒力基因数量相近，但 8 个耐药基因和 19 个毒力基因的序列在禽肉和畜肉之间存在显著差异。这些分离株的系统发育和共线性分析显示，同一种肉类中的分离株具有相似的基因组。这些结果表明，禽肉和畜肉中李斯特菌的遗传变异存在明显差异。
