Nonaka M, Nakayama K, Yeul Y D, Takahashi M
J Immunol. 1986 Apr 15;136(8):2989-93.
The nucleotide sequence coding for sex-limited protein (Slp), the testosterone-regulated isotype of the fourth component of mouse complement (C4), has been determined from cloned genomic DNA and cDNA fragments. The complete deduced amino acid sequence for the single chain precursor protein of Slp (pro-Slp) consists of 1716 residues. The mature beta, alpha, and gamma subunits contain 654, 763, and 291 amino acids, respectively. One potential carbohydrate attachment site is predicted from the beta-chain, five for the alpha-chain, and none for the gamma-chain. From the comparison with the mouse C4 sequences, an extensive overall sequence homology, 96.0% in nucleotides and 94.2% in amino acids, is observed. Only one deletion/insertion event is recognized between C4 and Slp sequences: three residues near the Cls cleavage site are deleted from Slp. The distribution of cysteine residues is completely conserved between pro-Slp and pro-C4.
编码性限制蛋白（Slp）的核苷酸序列已从克隆的基因组DNA和cDNA片段中确定，Slp是小鼠补体第四成分（C4）的睾酮调节同种型。Slp单链前体蛋白（pro-Slp）完整的推导氨基酸序列由1716个残基组成。成熟的β、α和γ亚基分别包含654、763和291个氨基酸。预测β链有一个潜在的碳水化合物附着位点，α链有五个，γ链没有。通过与小鼠C4序列比较，观察到广泛的整体序列同源性，核苷酸水平为96.0%，氨基酸水平为94.2%。在C4和Slp序列之间仅识别到一个缺失/插入事件：Slp在Cls裂解位点附近缺失三个残基。pro-Slp和pro-C4之间半胱氨酸残基的分布完全保守。
