溶液核磁共振技术在膜蛋白研究中的应用：挑战与创新。
Solution NMR investigations of integral membrane proteins: Challenges and innovations.
机构信息
Department of Chemistry, University of Virginia, Charlottesville, VA, USA.
Department of Chemistry, University of Virginia, Charlottesville, VA, USA.
出版信息
Curr Opin Struct Biol. 2023 Oct;82:102654. doi: 10.1016/j.sbi.2023.102654. Epub 2023 Aug 3.
Abstract
Compared to soluble protein counterparts, the understanding of membrane protein stability, solvent interactions, and function are not as well understood. Recent advancements in labeling, expression, and stabilization of membrane proteins have enabled solution nuclear magnetic resonance spectroscopy to investigate membrane protein conformational states, ligand binding, lipid interactions, stability, and folding. This review highlights these advancements and new understandings and provides examples of recent applications.
摘要
与可溶性蛋白相比，人们对膜蛋白的稳定性、溶剂相互作用和功能的了解还不够深入。近年来，在膜蛋白的标记、表达和稳定化方面的进展，使得溶液核磁共振波谱学能够研究膜蛋白的构象状态、配体结合、脂质相互作用、稳定性和折叠。本文重点介绍了这些进展和新的认识，并提供了最近应用的实例。
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