Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
Food Chem. 2024 Jan 1;430:137062. doi: 10.1016/j.foodchem.2023.137062. Epub 2023 Jul 29.
Sensitive detection strategies for enzymes and their inhibitors not only have critical significance in clinical diagnosis but can be extended to food and environment analysis. Here, bimetallic AgPd alloy nanoflowers were synthesized using carbon dots as the reductant via a two-step reduction method. The AgPd bimetallic nanoflowers exhibit ascorbate oxidase mimetic activity, which can rapidly catalyze the oxidization of ascorbic acid to dehydroascorbic acid (DHA). The DHA can then react with o-phenylenediamine (OPD) to produce 3-(1, 2-dihydroxyethyl)furo[3,4-b] quinoxalin-1(3H)-one (DFQ), accompanying with the characteristic fluorescence peak at 440 nm for DFQ. Alkaline phosphatase catalyzes l-ascorbyl-2-phosphate to generate AA, while organic phosphorus pesticides (OPPs) such as chlorpyrifos can inhibit the activity of alkaline phosphatase. By utilizing the enzyme-nanozyme tandem catalytic reaction, the fluorescence of the system varies in the presence of different concentrations of ALP or OPPs, which can be used to detect ALP in serum and OPPs in vegetables and fruits.
用于酶及其抑制剂的灵敏检测策略不仅在临床诊断中具有重要意义，而且可以扩展到食品和环境分析中。在这里，使用碳点作为还原剂，通过两步还原法合成了双金属 AgPd 合金纳米花。AgPd 双金属纳米花表现出抗坏血酸氧化酶模拟活性，可快速催化抗坏血酸氧化为脱氢抗坏血酸（DHA）。然后，DHA 可以与邻苯二胺（OPD）反应生成 3-(1,2-二羟乙基)呋喃[3,4-b]喹喔啉-1(3H)-酮（DFQ），同时伴随 DFQ 在 440nm 处的特征荧光峰。碱性磷酸酶催化 l-抗坏血基-2-磷酸生成 AA，而有机磷农药（OPPs）如毒死蜱可以抑制碱性磷酸酶的活性。通过利用酶-纳米酶串联催化反应，在存在不同浓度的 ALP 或 OPPs 时，系统的荧光会发生变化，可用于检测血清中的 ALP 和蔬菜、水果中的 OPPs。
