Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, PR China.
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, PR China.
Talanta. 2024 May 15;272:125704. doi: 10.1016/j.talanta.2024.125704. Epub 2024 Jan 30.
Herein, we successfully synthesized two-dimensional iron-doped carbon-based nanosheets (Fe-N CS) with catalase-like activity through doping Fe into Zn MOF and introducing graphitic CN (g-CN). The interaction of the Fe-N CS with hydrogen peroxide could generated abundant reactive oxygen species (ROS) and further oxidize o-Phenylenediamine (OPD) to 2,3-diaminophenazine (DAP) which has constant fluorescence at 560 nm. Ascorbic acid (AA) could be generated via the hydrolysis reaction between alkaline phosphatase (ALP) and ascorbic acid 2-phosphate (AAP). AA can be oxidized to dehy-droascorbic acid (DHA) by ROS, and then combined with OPD to generate 3-(1,2-dihydroxyethyl)furo[3,4b]-quinoxaline (QXD) with fluorescence at 440 nm, which could increase as the concentration of AA enhanced. DHA could also be generated through oxidation of AA by ascorbate oxidase (AAO). Thus, by monitoring the fluorescence ratio (I/I), a ratiometric fluorescence biosensing platform for ALP and AAO was established with the linear ranges in 0.2-10 U/L and 1-60 U/L, respectively. The limit of detection for ALP and AAO were 0.12 U/L and 0.59 U/L. Furthermore, the biosensing platform was successfully applied for the detection of ALP and AAO activity in human serum samples. This work provides a potential tool for future biomedical diagnostics.
在此，我们成功地通过将 Fe 掺杂到 Zn MOF 中并引入石墨相氮化碳（g-CN），合成了具有类过氧化物酶活性的二维铁掺杂碳基纳米片（Fe-N CS）。Fe-N CS 与过氧化氢相互作用会产生大量的活性氧（ROS），并进一步将邻苯二胺（OPD）氧化为具有恒定荧光的 2,3-二氨基吩嗪（DAP）（在 560nm 处有荧光）。碱性磷酸酶（ALP）和抗坏血酸 1-磷酸酯（AAP）之间的水解反应会产生抗坏血酸（AA）。AA 可被 ROS 氧化为脱氢抗坏血酸（DHA），然后与 OPD 结合生成具有 440nm 荧光的 3-（1,2-二羟乙基）呋喃[3,4b]-喹喔啉（QXD），随着 AA 浓度的增加，荧光强度也随之增加。DHA 还可以通过 AAO 氧化 AA 生成。因此，通过监测荧光比值（I/I），建立了一种用于检测 ALP 和 AAO 的比率荧光生物传感平台，其线性范围分别为 0.2-10 U/L 和 1-60 U/L。ALP 和 AAO 的检测限分别为 0.12 U/L 和 0.59 U/L。此外，该生物传感平台成功地应用于人血清样品中 ALP 和 AAO 活性的检测。这项工作为未来的生物医学诊断提供了一种潜在的工具。
