Cordingley J S, Haddow W J, Nene V, Taylor D W
Mol Biochem Parasitol. 1986 Jan;18(1):73-88. doi: 10.1016/0166-6851(86)90052-6.
cDNA clone banks have been constructed in a plasmid vector (pJSC73) using mRNA isolated from adult worms and eggs of Schistosoma mansoni. These clone banks have been screened by message selection (hybrid release translation) and clones containing fragments of genes encoding schistosome antigens have been identified by immunoprecipitation of the corresponding mRNA in vitro translation product. Over 50 clones encoding schistosome antigens have been identified including those of the 100 000, 86 000, 28 000, and 27 000 molecular weight surface antigen precursors.
利用从曼氏血吸虫成虫和虫卵中分离的mRNA，在质粒载体（pJSC73）中构建了cDNA克隆文库。这些克隆文库已通过信息选择（杂交释放翻译）进行筛选，并且通过对相应mRNA体外翻译产物的免疫沉淀，鉴定出了含有编码血吸虫抗原基因片段的克隆。已鉴定出50多个编码血吸虫抗原的克隆，包括分子量为100000、86000、28000和27000的表面抗原前体的克隆。
