Bloch B, Popovici T, Chouham S, Kowalski C
Neurosci Lett. 1986 Feb 14;64(1):29-34. doi: 10.1016/0304-3940(86)90658-0.
The messenger RNA coding for preproenkephalin A (PPA) has been detected in tissue sections of the rat brain and adrenal by using two rat PPA cDNAs labeled with 32P or 35S as probes. In the brain, neurons were labeled in areas known to correspond to sites of synthesis of enkephalins, including the caudate-putamen, the nucleus accumbens, the olfactory cortex, the hypothalamus, the brainstem and the granular layer of the cerebellum. The presence of the PPA mRNA in the normal rat adrenal medulla shows transcription of the PPA gene in such cells despite the absence of enkephalin immunoreactivity in them. These results demonstrate in situ hybridization as an efficient technique to detect the site of synthesis of PPA.
利用两个用³²P或³⁵S标记的大鼠前脑啡肽原A（PPA）cDNA作为探针，在大鼠脑和肾上腺的组织切片中检测到了编码PPA的信使RNA。在脑中，已知与脑啡肽合成部位相对应的区域的神经元被标记，包括尾状核 - 壳核、伏隔核、嗅觉皮层、下丘脑、脑干和小脑颗粒层。正常大鼠肾上腺髓质中PPA mRNA的存在表明，尽管这些细胞中没有脑啡肽免疫反应性，但PPA基因在这些细胞中仍有转录。这些结果证明原位杂交是检测PPA合成部位的有效技术。
