Wang Y, Yang Z W, Wang Q W, Xu Y Z, Liu X Y, Xu J F, Chen C H
Nucleic Acids Res. 1986 Mar 25;14(6):2699-706. doi: 10.1093/nar/14.6.2699.
The course of the activation of 3'-acetylthymidine 5'-phosphate by TPS and DCC were followed up by 31P FT nmr. The fact that the "metaphosphate" (delta-5.1) first becomes detectable only at later stage of the activation and does coexist with pyrophosphate and triphosphate suggests that the pyridinium derivative of "metaphosphate" is most probably not directly formed from the hypothetical mixed anhydride or active "pseudourea" right at the beginning of the reaction of pdTac with TPS or DCC, but rather formed at later stage of the activation reaction from the degradation of the pyro- and triphosphates by the activating agent. The mixed anhydride or the active "pseudourea" is most possibly the active key intermediate.
通过31P傅里叶变换核磁共振对胸苷-3'-磷酸-5'-乙酰酯（3'-acetylthymidine 5'-phosphate）被胸苷磷酸化酶（TPS）和二环己基碳二亚胺（DCC）激活的过程进行了跟踪。“偏磷酸酯”（δ-5.1）仅在激活的后期才首次可检测到，并且与焦磷酸和三磷酸共存，这一事实表明，“偏磷酸酯”的吡啶鎓衍生物很可能不是在pdTac与TPS或DCC反应开始时直接由假设的混合酸酐或活性“假脲”形成的，而是在激活反应的后期由激活剂对焦磷酸和三磷酸的降解形成的。混合酸酐或活性“假脲”很可能是活性关键中间体。
