State Key Laboratory of Swine and Poultry Breeding Industry, Farm Animal Genetic Resource Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Theriogenology. 2023 Oct 15;210:244-250. doi: 10.1016/j.theriogenology.2023.07.026. Epub 2023 Jul 22.
To investigate the relationship between polyamine metabolism and reproductive hormones in ovarian follicles of Sichuan white geese, follicle polyamine content and reproductive hormone levels and gene expressions related to polyamine metabolism, steroidogenesis and steroid hormone receptors were detected by HPLC, ELISA and RT-qPCR. The results showed that the overall trend of spermidine and spermine levels increased first and then decreased as increasing follicle size, with the highest level in F3 and F5 follicles (P < 0.05). Putrescine and 17β-estradiol (E2) levels in hierarchical follicles were significantly lower than those in prehierarchical follicles (P < 0.05). Progesterone (P4) first increased and then decreased, with the highest level in the F5 follicle (P < 0.05). The expression levels of estrogen receptor 1 (ER1) showed an overall increase as increasing follicle size (except in F3 follicles), while estrogen receptor 2 (ER2) in hierarchical follicles was significantly lower than that in the prehierarchical follicles (P < 0.05). In addition, the overall expression level of progesterone receptor (PR) decreased, with no significant differences among F1, F2 and F3 follicles (P > 0.05). Yolk putrescine contents were positively correlated with yolk E2 concentrations and PR expression levels (P < 0.05), A significant positive correlation of spermidine levels with yolk P4 concentrations and PR expressions was also observed, as well as the spermine levels with yolk P4 concentrations (P < 0.05). In summary, polyamines were involved in the regulation of follicular development in geese, and this regulation played a role in affecting steroidogenesis and the expression of genes related to hormone receptors.
为了研究四川白鹅卵巢卵泡中多胺代谢与生殖激素的关系，采用 HPLC、ELISA 和 RT-qPCR 检测卵泡多胺含量及与多胺代谢、甾体生成和甾体激素受体相关的生殖激素水平和基因表达。结果表明，随着卵泡大小的增加，精脒和精胺的总体趋势为先增加后减少，在 F3 和 F5 卵泡中达到最高水平（P<0.05）。分级卵泡中的腐胺和 17β-雌二醇（E2）水平明显低于前分级卵泡（P<0.05）。孕酮（P4）先增加后减少，在 F5 卵泡中达到最高水平（P<0.05）。雌激素受体 1（ER1）的表达水平随着卵泡大小的增加呈总体增加趋势（除 F3 卵泡外），而分级卵泡中的雌激素受体 2（ER2）明显低于前分级卵泡（P<0.05）。此外，孕激素受体（PR）的整体表达水平下降，F1、F2 和 F3 卵泡之间无显著差异（P>0.05）。卵黄腐胺含量与卵黄 E2 浓度和 PR 表达水平呈正相关（P<0.05），精脒水平与卵黄 P4 浓度和 PR 表达水平也呈显著正相关，精胺水平与卵黄 P4 浓度呈正相关（P<0.05）。综上所述，多胺参与了鹅卵泡发育的调节，这种调节作用在影响甾体生成和与激素受体相关基因的表达中发挥了作用。
