Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, School of Petrochemical Engineering, Changzhou University, Changzhou, Jiangsu, 213164, PR China.
Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, School of Petrochemical Engineering, Changzhou University, Changzhou, Jiangsu, 213164, PR China.
Biosens Bioelectron. 2023 Oct 15;238:115551. doi: 10.1016/j.bios.2023.115551. Epub 2023 Jul 26.
The development of innovative and efficient strategy is of paramount importance for near-infrared (NIR) electrochemiluminescence (ECL) sensing, which can substantially promote ECL detection in a wide range of situations. Herein, the inner filter effect (IFE) strategy was designed to construct an ultrasensitive NIR ECL biosensor based on the well-matched AgBr nanocrystals (NCs) decorated nitrogen-doped TiC MXene nanocomposites (AgBr-N-TiC) and hydrated defective tungsten oxide nanosheets (dWO•HO). Specifically, the AgBr-N-TiC nanocomposites displayed extremely effective NIR ECL emission because N-doping could accelerate electron transfer and boost the red-shift of the ECL spectrum. The nonmetallic plasmon dWO•HO was used as an absorber due to its facile tuning of the spectra overlap and higher molar extinction coefficients. Time-resolved emission decay curves proved that the decreased ECL intensity was ascribed to the IFE-based steady quenching mechanism. With the support of tetracycline (TC) aptamer and the complementary DNA chain, the fabricated NIR ECL-IFE biosensor performed a wide linear range of 100 nM ∼ 10 fM with a low detection limit of 2.2 fM (S/N = 3), and it exhibited excellent stability, sensitivity, and reproducibility, so as to be applied to real samples. This strategy opens a new avenue to constructing an efficient NIR ECL-IFE system and shows excellent practical potential in actual sample analysis.
开发创新且高效的策略对于近红外（NIR）电化学发光（ECL）传感至关重要，这可以极大地促进各种情况下的 ECL 检测。在此，设计了内滤效应（IFE）策略，以基于匹配良好的 AgBr 纳米晶体（NCs）修饰的氮掺杂 TiC MXene 纳米复合材料（AgBr-N-TiC）和水合缺陷态氧化钨纳米片（dWO·HO）构建超灵敏的 NIR ECL 生物传感器。具体而言，AgBr-N-TiC 纳米复合材料表现出极其有效的 NIR ECL 发射，因为 N 掺杂可以加速电子转移并促进 ECL 光谱的红移。由于其易于调整光谱重叠和更高的摩尔消光系数，非金属等离子体 dWO·HO 被用作吸收剂。时间分辨发射衰减曲线证明，ECL 强度的降低归因于基于 IFE 的稳态猝灭机制。在四环素（TC）适体和互补 DNA 链的支持下，所构建的 NIR ECL-IFE 生物传感器表现出宽线性范围为 100 nM ～ 10 fM，检测限低至 2.2 fM（S/N = 3），并且表现出出色的稳定性、灵敏度和重现性，可用于实际样品分析。该策略为构建高效的 NIR ECL-IFE 系统开辟了新途径，并在实际样品分析中显示出了优异的实际潜力。
