Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, School of Petrochemical Engineering, Changzhou University, Changzhou, Jiangsu, 213164, PR China.
Oakland International Associated Laboratory, School of Photoelectric Engineering, Changzhou Institute of Technology, Changzhou, Jiangsu, 213032, PR China; Jiangsu Key Laboratory of Materials Surface Science and Technology, Changzhou University, Changzhou, Jiangsu, 213164, PR China.
Biosens Bioelectron. 2022 Mar 15;200:113917. doi: 10.1016/j.bios.2021.113917. Epub 2021 Dec 27.
Toxic-free and easily accessible electrochemiluminescence (ECL) emitter/luminophore with near-infrared (NIR) emission is highly anticipated for ECL biosensor evolution. In this study, well-dispersed AgBr nanocrystals (NCs) decorated TiC MXene nanocomposites (TiC-AgBrNCs) were prepared using a simple wet chemical technique and demonstrated highly efficient NIR ECL emission. For the first time, TiC-AgBrNCs displayed wavelength-tunable ECL emission with varied TiC contents. Interestingly, further experimental data revealed that the ECL emission wavelength of TiC-AgBrNCs red-shifted from 550 to 665 nm as TiC content increased, which can be attributed to the surface-defect effect generated by the oxygen-containing functional groups in TiC MXene. In particular, the ECL emission at 665 nm of TiC-AgBrNCs nanocomposites not only revealed a 3.5 times increased ECL intensity but also a more stable ECL signal compared to pure AgBr NCs. As a proof of concept, a direct-type NIR ECL aptasensor with signal-on strategy was constructed with the TiC-AgBrNCs nanocomposites as an ECL platform and enrofloxacin (ENR) as a model analyte. The NIR ECL aptasensor exhibited high sensitivity, a wide linear range from 1.0 × 10 mol/L to 1.0 × 10 mol/L and a low detection limit (5.97 × 10 mol/L). This research offered a viable alternative way for producing toxic-free and efficient near-infrared ECL luminophores in bioanalysis and wavelength-tuning light-emitting devices.
无毒且易于获得的具有近红外（NIR）发射的电致化学发光（ECL）发射器/发光体，对于 ECL 生物传感器的发展非常重要。在这项研究中，使用简单的湿化学技术制备了分散良好的 AgBr 纳米晶体（NCs）修饰的 TiC MXene 纳米复合材料（TiC-AgBrNCs），并证明了其具有高效的 NIR ECL 发射。首次，TiC-AgBrNCs 显示出具有不同 TiC 含量的可调谐 ECL 发射。有趣的是，进一步的实验数据表明，TiC-AgBrNCs 的 ECL 发射波长从 550nm 红移到 665nm，随着 TiC 含量的增加，这可以归因于 TiC MXene 中含氧官能团产生的表面缺陷效应。特别是，TiC-AgBrNCs 纳米复合材料的 665nm ECL 发射不仅显示出 3.5 倍的增强 ECL 强度，而且与纯 AgBr NCs 相比，具有更稳定的 ECL 信号。作为概念验证，使用 TiC-AgBrNCs 纳米复合材料作为 ECL 平台和恩诺沙星（ENR）作为模型分析物，构建了具有信号开启策略的直接型 NIR ECL 适体传感器。NIR ECL 适体传感器表现出高灵敏度，从 1.0×10-9 mol/L 到 1.0×10-7 mol/L 的宽线性范围和低检测限（5.97×10-9 mol/L）。这项研究为生物分析和波长调谐发光器件中生产无毒且高效的近红外 ECL 发光体提供了一种可行的替代方法。
