利用 FeO-GO-Agarose-Chitosan 复合材料对鸡蛋样品中氨基糖苷类残留进行磁固相萃取。

Magnetic solid phase extraction of aminoglycosides residue in chicken egg samples using FeO-GO-Agarose-Chitosan composite.

机构信息

Institute of Chemical Sciences, Bahauddin Zakariya University, Multan 60800, Pakistan.

Department of Chemistry, College of Science, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia.

出版信息

Food Chem. 2024 Jan 1;430:137092. doi: 10.1016/j.foodchem.2023.137092. Epub 2023 Aug 2.

DOI:10.1016/j.foodchem.2023.137092

PMID:37544153

Abstract

Difficulties in identification of drug residues in food products arise due to their trace amounts in complex matrices. An eco-friendly and low-cost agarose-chitosan-magnetic graphene oxide based adsorbent was synthesized and employed for determination of aminoglycosides from chicken egg samples through HPLC. Synthesized adsorbent was characterized by SEM, FTIR, XRD, and VSM. Among two investigated aminoglycosides, streptomycin was derivatized with ninhydrin while gentamicin was detected without its derivatization. Impact of experimental variables such as adsorbent dose, extraction time, temperature, pH, and analyte concentration on extraction efficiency was investigated. Statistical analysis for determination of streptomycin and gentamicin demonstrated excellent linearity in the range of 0.2-1.6 µg kg, LOQ of 0.3 and 0.6 µg kg for streptomycin and gentamicin, respectively and LOD of 0.1 and 0.19 µg kg for streptomycin and gentamicin, respectively with RSD of 2.5% and recoveries up to 94%. Regeneration studies revealed that composite film can be used four times without considerable reduction in its extraction efficiency.

摘要

由于痕量药物残留存在于复杂基质中，因此在食品中鉴定药物残留存在困难。本研究合成了一种环保且低成本的琼脂糖-壳聚糖-磁性氧化石墨烯基吸附剂，并通过高效液相色谱法（HPLC）用于测定鸡蛋样品中的氨基糖苷类药物。通过 SEM、FTIR、XRD 和 VSM 对合成的吸附剂进行了表征。在所研究的两种氨基糖苷类药物中，链霉素用茚三酮衍生化，而庆大霉素则无需衍生化即可检测到。考察了吸附剂用量、提取时间、温度、pH 值和分析物浓度等实验变量对提取效率的影响。对链霉素和庆大霉素的测定进行了统计学分析，结果表明，在 0.2-1.6μg·kg 的范围内具有良好的线性关系，链霉素和庆大霉素的 LOQ 分别为 0.3 和 0.6μg·kg，LOD 分别为 0.1 和 0.19μg·kg，相对标准偏差（RSD）为 2.5%，回收率高达 94%。再生研究表明，复合膜在不明显降低提取效率的情况下可以重复使用四次。