Cell Factory Research Center, Korea Research Institute of Bioscience & Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea; Department of Environmental Biotechnology, KRIBB School of Biotechnology, University of Science and Technology, Daejeon 34113, Republic of Korea.
Cell Factory Research Center, Korea Research Institute of Bioscience & Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
Sci Total Environ. 2023 Dec 1;902:165888. doi: 10.1016/j.scitotenv.2023.165888. Epub 2023 Aug 5.
Although nutrient availability is widely recognized as the driving force behind Microcystis blooms, identifying the microorganisms that play a pivotal role in their formation is a challenging task. Our understanding of the contribution of bacterial communities to the development of Microcystis blooms remains incomplete, despite the fact that the relationship between Microcystis and bacterial communities has been extensively investigated. Most studies have focused on their interaction for a single year rather than for multiple years. To determine key bacteria crucial for the formation of Microcystis blooms, we collected samples from three sites in the Daechung Reservoir (Chuso, Hoenam, and Janggye) over three years (2017, 2019, and 2020). Our results indicated that Microcystis bloom-associated bacterial communities were more conserved across stations than across years. Bacterial communities could be separated into modules corresponding to the different phases of Microcystis blooms. Dolichospermum and Aphanizomenon belonged to the same module, whereas the module of Microcystis was distinct. The microbial recurrent association network (MRAN) showed that amplicon sequence variants (ASVs) directly linked to Microcystis belonged to Pseudanabaena, Microscillaceae, Sutterellaceae, Flavobacterium, Candidatus Aquiluna, Bryobacter, and DSSD61. These ASVs were also identified as key indicators of the bloom stage, indicating that they were fundamental biological elements in the development of Microcystis blooms. Overall, our study highlights that, although bacterial communities change annually, they continue to share core ASVs that may be crucial for the formation and maintenance of Microcystis blooms.
尽管营养物质的可利用性被广泛认为是引发微囊藻水华的驱动力，但确定在其形成过程中起关键作用的微生物却是一项具有挑战性的任务。尽管已经广泛研究了微囊藻与细菌群落之间的关系，但我们对细菌群落对微囊藻水华发展的贡献的理解仍然不完整。大多数研究都集中在单一年份的相互作用上，而不是多年的相互作用。为了确定对微囊藻水华形成至关重要的关键细菌，我们在三年（2017 年、2019 年和 2020 年）中从大仓水库的三个地点（楚松、湖南和江亭）采集了样本。我们的结果表明，与微囊藻水华相关的细菌群落在站点之间比在年份之间更保守。细菌群落可以分为与微囊藻水华不同阶段相对应的模块。念珠藻和鱼腥藻属于同一个模块，而微囊藻的模块则不同。微生物反复关联网络（MRAN）显示，与微囊藻直接相关的扩增子序列变体（ASVs）属于假鱼腥藻属、微丝菌科、Sutterellaceae、黄杆菌属、Aquiluna 候选菌属、Bryobacter 和 DSSD61。这些 ASVs 也被鉴定为水华阶段的关键指标，表明它们是微囊藻水华发展的基本生物要素。总的来说，我们的研究表明，尽管细菌群落每年都在变化，但它们仍然共享可能对微囊藻水华的形成和维持至关重要的核心 ASVs。
