Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.
Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju 63243, Republic of Korea.
J Microbiol Biotechnol. 2024 Nov 28;34(11):2245-2251. doi: 10.4014/jmb.2405.05026. Epub 2024 Sep 29.
A novel Gram-negative bacterial strain, 20NA77.7, was isolated from fresh water of the Nakdong River. Strain 20NA77.7 shared the highest similarity with GPTSA100-9 (97.91%) and DSM 27078 (96.24%) in the 16S rRNA gene sequence. The digital DNA-DNA hybridization and average nucleotide identity values for strain 20NA77.7 with species were below 20.8% and 77.33%, respectively. The major fatty acids of strain 20NA77.7 were identified as iso-C, iso-C, iso-C G, anteiso-C, iso-C 3OH, and iso-C 3OH. Strain 20NA77.7 contained phosphatidylethanolamine, one unidentified aminolipid, and three unidentified lipids as polar lipids and menaquinone-6 as menaquinone. The polyphasic evidence supports the classification of strain 20NA77.7 as a novel species belonging to the genus , for which the name is proposed. The type strain is 20NA77.7 (= KCTC 102000 = LMG 33137).
一株新的革兰氏阴性细菌菌株 20NA77.7 从南洞江的淡水中分离得到。菌株 20NA77.7 在 16S rRNA 基因序列上与 GPTSA100-9（97.91%）和 DSM 27078（96.24%）的相似度最高。菌株 20NA77.7 与种之间的数字 DNA-DNA 杂交和平均核苷酸同一性值分别低于 20.8%和 77.33%。菌株 20NA77.7 的主要脂肪酸为 iso-C、iso-C、iso-C G、anteiso-C、iso-C 3OH 和 iso-C 3OH。菌株 20NA77.7 含有磷脂酰乙醇胺、一种未鉴定的胺脂和三种未鉴定的脂类作为极性脂类，以及menaquinone-6 作为menaquinone。多相证据支持将菌株 20NA77.7 分类为属于属的一个新种，建议使用名称。模式株为 20NA77.7（=KCTC 102000=LMG 33137）。
