Liang Li, Zhang Guosheng, Dai Xuening, Li Weiying
State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai, 200092, PR China; College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, PR China.
State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai, 200092, PR China; College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, PR China; Key Laboratory of Yangtze River Water Environment of the Ministry of Education, Tongji University, Shanghai, 200092, PR China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai, 200092, PR China.
Environ Res. 2023 Nov 1;236(Pt 2):116829. doi: 10.1016/j.envres.2023.116829. Epub 2023 Aug 5.
Antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) have drawn much more attention due to their high risk on human health and ecosystem. In this study, the performance of sulfidated nanoscale zero-valent iron (S-nZVI)/periodate (PI) system toward ARB inactivation and ARGs removal was systematically investigated. The S-nZVI/PI system could realize the complete inactivation of 1 × 10 CFU/mL kanamycin, ampicillin, and tetracycline-resistant E. coli HB101 within 40 min, meanwhile, possessed the ability to remove the intracellular ARGs (iARGs) (including aphA, tetA, and tnpA) carried by E. coli HB101. Specifically, the removal of aphA, tetA, and tnpA by S-nZVI/PI system after 40 min reaction was 0.31, 0.47, and 0.39 logcopies/mL, respectively. The reactive species attributed to the E. coli HB101 inactivation were HO and O, which could cause the destruction of E. coli HB101 morphology and enzyme system (such as superoxide dismutase and catalase), the loss of intracellular substances, and the damage of iARGs. Moreover, the influence of the dosage of PI and S-nZVI, the initial concentration of E. coli HB101, as well as the co-existing substance (such as HCO, NO, and humic acid (HA)) on the inactivation of E. coli HB101 and its corresponding iARGs removal was also conducted. It was found that the high dosage of PI and S-nZVI and the low concentration of E. coli HB101 could enhance the disinfection performance of S-nZVI/PI system. The presence of HCO, NO, and HA in S-nZVI/PI system showed inhibiting role on the inactivation of E. coli HB101 and its corresponding iARGs removal. Overall, this study demonstrates the superiority of S-nZVI/PI system toward ARB inactivation and ARGs removal.
抗生素抗性细菌（ARB）和抗生素抗性基因（ARGs）因其对人类健康和生态系统的高风险而备受关注。在本研究中，系统地研究了硫化纳米零价铁（S-nZVI）/过碘酸盐（PI）体系对ARB灭活和ARGs去除的性能。S-nZVI/PI体系能够在40分钟内实现对1×10 CFU/mL卡那霉素、氨苄青霉素和四环素抗性大肠杆菌HB101的完全灭活，同时具备去除大肠杆菌HB101携带的细胞内ARGs（包括aphA、tetA和tnpA）的能力。具体而言，反应40分钟后，S-nZVI/PI体系对aphA、tetA和tnpA的去除率分别为0.31、0.47和0.39 log拷贝/mL。导致大肠杆菌HB101失活的活性物种是HO和O，它们可导致大肠杆菌HB101形态和酶系统（如超氧化物歧化酶和过氧化氢酶）的破坏、细胞内物质的损失以及iARGs的损伤。此外，还研究了PI和S-nZVI的用量、大肠杆菌HB101的初始浓度以及共存物质（如HCO、NO和腐殖酸（HA））对大肠杆菌HB101灭活及其相应iARGs去除的影响。结果发现，高剂量的PI和S-nZVI以及低浓度的大肠杆菌HB101可增强S-nZVI/PI体系的消毒性能。S-nZVI/PI体系中HCO、NO和HA的存在对大肠杆菌HB101的灭活及其相应iARGs的去除表现出抑制作用。总体而言，本研究证明了S-nZVI/PI体系在ARB灭活和ARGs去除方面的优越性。
