National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Faculty of Environment and Life Sciences, Beijing University of Technology, Beijing 100124, China.
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Faculty of Environment and Life Sciences, Beijing University of Technology, Beijing 100124, China.
J Hazard Mater. 2022 Feb 5;423(Pt A):126866. doi: 10.1016/j.jhazmat.2021.126866. Epub 2021 Aug 8.
Antibiotic resistant bacteria (ARB) and the antibiotic resistance genes (ARGs) dissemination via plasmid-mediated conjugation have attracted considerable attentions. In this research, sulfidated nanoscale zerovalent iron (S-nZVI)/peroxymonosulfate (PMS) and S-nZVI/peroxydisulfate (PDS) process were investigated to inactivate ARB (Escherichia coli DH5α with RP4 plasmid, Pseudomonas. HLS-6 contains sul1 and intI1 on genome DNA sequence). S-nZVI/PMS system showed higher efficiency than S-nZVI/PDS on ARB inactivation. Thus, the optimal condition 28 mg/L S-nZVI coupled with 153.7 mg/L (0.5 mM) PMS was applied to remove both intracellular ARGs (iARGs) and ARB. The oxidative damage of ARB cell was systemically studied by cell viability, intracellular Mg levels, the changes of extracellular and internal structure, integrity of cell walls and membranes and enzymatic activities. S-nZVI/PMS effectively inactivated ARB (~7.32 log) within 15 min. These effects were greatly higher than those achieved individually. Moreover, removal efficiencies of iARGs sul1, intI1 and tetA were 1.52, 1.79 and 1.56 log, respectively. These results revealed that S-nZVI and PMS have a synergistic effect against ARB and iARGs. The regrowth assays illustrated that the ARB were effectively inactivated. By verifying the inhibitory impacts of S-nZVI/PMS treatment on conjugation transfer, this work highlights a promising alternative technique for inhibiting the horizontal gene transfer.
耐抗生素细菌（ARB）和抗生素耐药基因（ARGs）通过质粒介导的接合传播引起了相当大的关注。在这项研究中，研究了硫化纳米零价铁（S-nZVI）/过一硫酸盐（PMS）和 S-nZVI/过二硫酸盐（PDS）工艺，以灭活 ARB（携带 RP4 质粒的大肠杆菌 DH5α，基因组 DNA 序列上含有 sul1 和 intI1 的假单胞菌 HLS-6）。S-nZVI/PMS 系统在灭活 ARB 方面比 S-nZVI/PDS 系统更有效。因此，采用了最佳条件 28 mg/L S-nZVI 与 153.7 mg/L（0.5 mM）PMS 结合，以去除细胞内 ARGs（iARGs）和 ARB。通过细胞活力、细胞内镁水平、细胞外和内部结构变化、细胞壁和细胞膜完整性以及酶活性，系统研究了 ARB 细胞的氧化损伤。S-nZVI/PMS 在 15 分钟内有效灭活 ARB（~7.32 log）。这些效果远高于单独使用时的效果。此外，iARGs sul1、intI1 和 tetA 的去除效率分别为 1.52、1.79 和 1.56 log。这些结果表明 S-nZVI 和 PMS 对 ARB 和 iARGs 具有协同作用。再生试验表明，ARB 被有效灭活。通过验证 S-nZVI/PMS 处理对接合转移的抑制影响，这项工作突出了一种抑制水平基因转移的有前途的替代技术。
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