Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China; Key Laboratory of Technology and Model for Cyclic Utilization from Agricultural Resources, Ministry of Agriculture and Rural Affairs, Beijing 100125, China.
Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.
Bioresour Technol. 2023 Nov;387:129633. doi: 10.1016/j.biortech.2023.129633. Epub 2023 Aug 5.
This study aims to reveal the underlying mechanisms of mature compost addition for improving organic waste composting. Composting experiments and metagenomic analysis were conducted to elucidate the role of mature compost addition to regulate microbial metabolisms and physiological behaviors for composting amelioration. Mature compost with or without inactivation pretreatment was added to the composting of kitchen and garden wastes at 0%, 5%, 10%, 15%, and 20% (by wet weight) for comparison. Results show that mature compost promoted pyruvate metabolism, tricarboxylic acid (TCA) cycle, and oxidative phosphorylation to produce heat and energy to accelerate temperature increase for composting initiation and biological contaminant removal (>78%) for pasteurization. Energy requirement drives bacterial chemotactic motility towards nutrient-rich regions to sustain organic biodegradation. Nevertheless, when NADH formation exceeded NAD regeneration in oxidative phosphorylation, TCA cycle was restrained to limit continuous temperature increase and recover high intracellular NAD/NADH ratio to secure stable oxidation reactions.
本研究旨在揭示成熟堆肥添加改善有机废物堆肥的潜在机制。通过堆肥实验和宏基因组分析，阐明了成熟堆肥添加调节微生物代谢和生理行为以改善堆肥的作用。将具有或不具有失活预处理的成熟堆肥以 0%、5%、10%、15%和 20%（按湿重计）的比例添加到厨房和花园废物的堆肥中进行比较。结果表明，成熟堆肥促进丙酮酸代谢、三羧酸（TCA）循环和氧化磷酸化，产生热量和能量，以加速堆肥启动的温度升高，并实现生物污染物去除（>78%）以进行巴氏消毒。能量需求驱使细菌向营养丰富的区域进行趋化运动，以维持有机生物降解。然而，当氧化磷酸化中 NADH 的形成超过 NAD 的再生时，TCA 循环受到限制，以限制温度的持续升高，并恢复高细胞内 NAD/NADH 比，以确保稳定的氧化反应。
