School of Traditional Chinese Medicine, Naval Medical University, Shanghai 200433, China.
School of Traditional Chinese Medicine, Naval Medical University, Shanghai 200433, China; Department of Dermatology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
J Integr Med. 2023 Sep;21(5):487-495. doi: 10.1016/j.joim.2023.07.002. Epub 2023 Jul 25.
This study tests the efficacy of Bletilla striata polysaccharide (BSP), carboxymethyl chitosan (CMC), baicalin (BA) and silver titanate (ST) in a wound dressings to fight infection, promote healing and provide superior biocompatibility.
The antibacterial activity of BA and ST was evaluated in vitro using the inhibition zone method. BA/ST/BSP/CMC porous sponge dressings were prepared and characterized. The biocompatibility of BA/ST/BSP/CMC was assessed using the cell counting kit-8 assay. The therapeutic effect of BA/ST/BSP/CMC was further investigated using the dorsal skin burn model in Sprague-Dawley rats.
The wound dressing had good antibacterial activity against Escherichia coli and Staphylococcus aureus through BA and ST, while the combination of BSP and CMC played an important role in promoting wound healing. The BA/ST/BSP/CMC porous sponge dressings were prepared using a freeze-drying method with the concentrations of BA and ST at 20 and 0.83 mg/mL, respectively, and the optimal ratio of 5% BSP to 4% CMC was 1:3. The average porosity, water absorption and air permeability of BA/ST/BSP/CMC porous sponge dressings were measured to be 90.43%, 746.1% and 66.60%, respectively. After treatment for 3 and 7 days, the healing rates of the BA/ST/BSP/CMC group and BA/BSP/CMC group were significantly higher than those of the normal saline (NS) group and silver sulfadiazine (SSD) group (P < 0.05). Interleukin-1β expression in the BA/ST/BSP/CMC group at 1 and 3 days was significantly lower than that in the other three groups (P < 0.05). After being treated for 3 days, vascular endothelial growth factor expression in the BA/BSP/CMC group and BA/ST/BSP/CMC group was significantly higher than that in the NS group and SSD group (P < 0.05). Inspection of histological sections showed that the BA/ST/BSP/CMC group and BA/BSP/CMC group began to develop scabbing and peeling of damaged skin after 3 days of treatment, indicating accelerated healing relative to the NS group and SSD group.
The optimized concentration of BA/ST/BSP/CMC dressing was as follows: 6 mg BSP, 14.4 mg CMC, 0.5 mg ST and 12 mg BA. The BA/ST/BSP/CMC dressing, containing antibacterial constituents, was non-cytotoxic and effective in accelerating the healing of burn wounds, making it a promising candidate for wound healing. Please cite this article as: Gong YR, Zhang C, Xiang X, Wang ZB, Wang YQ, Su YH, Zhang HQ. Baicalin, silver titanate, Bletilla striata polysaccharide and carboxymethyl chitosan in a porous sponge dressing for burn wound healing. J Integr Med. 2023; 21(5): 487-495.
本研究旨在测试白芨多糖（BSP）、羧甲基壳聚糖（CMC）、黄芩苷（BA）和纳米银钛（ST）在伤口敷料中的功效，以抗感染、促进愈合并提供卓越的生物相容性。
采用抑菌圈法评价 BA 和 ST 的体外抗菌活性。制备并表征 BA/ST/BSP/CMC 多孔海绵敷料。使用细胞计数试剂盒-8 测定 BA/ST/BSP/CMC 的生物相容性。进一步通过 Sprague-Dawley 大鼠背部皮肤烧伤模型研究 BA/ST/BSP/CMC 的治疗效果。
BA 和 ST 的伤口敷料对大肠杆菌和金黄色葡萄球菌具有良好的抗菌活性，而 BSP 和 CMC 的组合在促进伤口愈合方面发挥了重要作用。BA/ST/BSP/CMC 多孔海绵敷料采用冷冻干燥法制备，BA 和 ST 的浓度分别为 20 和 0.83 mg/mL，BSP 和 CMC 的最佳比例为 1:3（5% BSP 对 4% CMC）。BA/ST/BSP/CMC 多孔海绵敷料的平均孔隙率、吸水率和透气率分别为 90.43%、746.1%和 66.60%。治疗 3 天和 7 天后，BA/ST/BSP/CMC 组和 BA/BSP/CMC 组的愈合率明显高于生理盐水（NS）组和磺胺嘧啶银（SSD）组（P < 0.05）。BA/ST/BSP/CMC 组在 1 天和 3 天时的白细胞介素-1β表达明显低于其他三组（P < 0.05）。治疗 3 天后，BA/BSP/CMC 组和 BA/ST/BSP/CMC 组的血管内皮生长因子表达明显高于 NS 组和 SSD 组（P < 0.05）。组织学切片检查显示，BA/ST/BSP/CMC 组和 BA/BSP/CMC 组在治疗 3 天后开始结痂和剥落受损皮肤，表明与 NS 组和 SSD 组相比，愈合速度加快。
优化的 BA/ST/BSP/CMC 敷料浓度为：6 mg BSP、14.4 mg CMC、0.5 mg ST 和 12 mg BA。含抗菌成分的 BA/ST/BSP/CMC 敷料无细胞毒性，能有效促进烧伤创面愈合，是一种有前途的创面愈合材料。请引用本文：龚玉蓉，张超，向祥，王智斌，王艳秋，苏贻豪，张宏旗。黄芩苷、纳米银钛、白芨多糖和羧甲基壳聚糖在多孔海绵敷料中的应用促进烧伤创面愈合。J 中西医结合杂志。2023;21(5):487-495.
