Department of Orthopedics, People's Hospital Yangxin County of Hubei Province, Huangshi 435200, China.
Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, China.
Biomed Res Int. 2021 Mar 30;2021:8872235. doi: 10.1155/2021/8872235. eCollection 2021.
To explore the effect and mechanism of the sponge dressing on the healing of refractory orthopedic wound, and the gelatin-Bletilla striata gum/Salvia miltiorrhiza nano Ag (GBS-Ag) sponge dressing was prepared.
GBS-Ag sponge dressing was prepared by the freeze-drying method. Twenty male SD rats were randomly divided into the control group (Ctrl group) and GBS-Ag group, with 10 rats in each group, and the rats in the two groups were established a model of back wound infection. The Ctrl group was treated with gauze, while the GBS-Ag group was treated with GBS-Ag sponge dressing. Wound healing rate, blood immune indexes, Ag content in each organ, morphological changes of wound, and expression of transforming growth factor-1 (TGF-1) in wound transformation were detected in the two groups of rats.
The mechanical properties of GBS-Ag sponge dressing were all in line with the standard, and it had good killing effect on the conventional strain after being incubated for 24 hours. Compared with the Ctrl group, the healing rate and lymphocyte percentage in the GBS-Ag group were significantly increased on day 4 and day 10 ( < 0.05), while the total number of white blood cells and the percentage of neutrophils were significantly decreased ( < 0.05). Compared with Ctrl group, the Ag content in liver, spleen, and kidney of rats in the GBS-Ag group was significantly increased ( < 0.05). The histological results showed that the Ctrl group lacked collagen fibers in the dermis, and the angiogenesis was not rich, accompanied by a large number of inflammatory cell infiltration. The epidermal repair of rats in the GBS-Ag group was complete and partially keratinized, the dermis was rich in collagen fibers, with elastic fibers and new blood vessels, inflammatory cells were rare, and new hair follicles and thick-walled blood vessels were also observed. The expression of TGF-1 protein in the wounds of rats in the GBS-Ag group was higher than that of the Ctrl group.
GBS-Ag sponge dressing had multiple effects of sterilization and promoting wound healing, and its mechanism may be related to promoting the TGF-1 protein expression.
探讨海绵敷料对难治性骨科创面愈合的作用及机制，并制备明胶-白及胶/丹参纳米银（GBS-Ag）海绵敷料。
采用冷冻干燥法制备 GBS-Ag 海绵敷料。将 20 只雄性 SD 大鼠随机分为对照组（Ctrl 组）和 GBS-Ag 组，每组 10 只，两组大鼠均建立背部伤口感染模型。Ctrl 组采用纱布治疗，GBS-Ag 组采用 GBS-Ag 海绵敷料治疗。检测两组大鼠的创面愈合率、血液免疫指标、各器官 Ag 含量、创面形态学变化及创面转化生长因子-1（TGF-1）表达。
GBS-Ag 海绵敷料的力学性能均符合标准，孵育 24 小时后对常规菌株具有良好的杀灭效果。与 Ctrl 组相比，GBS-Ag 组大鼠在第 4 天和第 10 天的愈合率和淋巴细胞百分比明显增加（<0.05），而白细胞总数和中性粒细胞百分比明显减少（<0.05）。与 Ctrl 组相比，GBS-Ag 组大鼠肝、脾、肾中的 Ag 含量明显增加（<0.05）。组织学结果显示，Ctrl 组真皮内缺乏胶原纤维，血管生成不丰富，伴有大量炎性细胞浸润。GBS-Ag 组大鼠的表皮修复完整，部分角化，真皮内富含胶原纤维，有弹性纤维和新血管，炎性细胞少见，还观察到新生毛囊和厚壁血管。GBS-Ag 组大鼠创面 TGF-1 蛋白表达高于 Ctrl 组。
GBS-Ag 海绵敷料具有杀菌和促进伤口愈合的多重作用，其机制可能与促进 TGF-1 蛋白表达有关。
