Seyedin S M, Thompson A Y, Bentz H, Rosen D M, McPherson J M, Conti A, Siegel N R, Galluppi G R, Piez K A
J Biol Chem. 1986 May 5;261(13):5693-5.
Comparison of the sequence of the N-terminal 30 amino acids of cartilage-inducing factor-A (CIF-A) from bovine demineralized bone with the corresponding sequence of human transforming growth factor-beta (TGF-beta) revealed 100% identity. Furthermore, CIF-A stimulated normal rat kidney fibroblasts to become anchorage-independent and form colonies in soft agar (in the presence of epidermal growth factor) in a manner similar to TGF-beta. Similarly, TGF-beta from human platelets induced rat muscle mesenchymal cells to differentiate and synthesize cartilage-specific macromolecules in a manner equivalent to CIF-A. These data show that CIF-A and TGF-beta are closely related or identical molecules and that these factors may be involved in cell differentiation including cartilage formation as the first step in endochondral bone formation.
将来自牛脱矿骨的软骨诱导因子A（CIF-A）N端30个氨基酸的序列与人类转化生长因子β（TGF-β）的相应序列进行比较，结果显示二者完全相同。此外，CIF-A能刺激正常大鼠肾成纤维细胞变得不依赖贴壁，并在软琼脂中（在表皮生长因子存在的情况下）形成集落，其方式与TGF-β相似。同样，来自人血小板的TGF-β能诱导大鼠肌肉间充质细胞分化并合成软骨特异性大分子，其方式与CIF-A相当。这些数据表明，CIF-A和TGF-β是密切相关或相同的分子，并且这些因子可能参与细胞分化，包括软骨形成，而软骨形成是软骨内骨形成的第一步。
