Laser and Photonics Research Center, University of Al-Hamdaniya, Nineveh, Iraq.
Department of Biology, College of Science, University of Basrah, Basrah, Iraq.
Open Vet J. 2023 Jun;13(6):765-771. doi: 10.5455/OVJ.2023.v13.i6.11. Epub 2023 Jun 17.
The fungi species are widespread airborne contaminants and are thought to be natural occupants of human skin, lungs, urine, and feces. Therefore, , , and are three of the most prevalent species.
This study aims to isolate from the rumen fluid of cows in the province of Mosul and to determine how laser light irradiation affects the growth and morphological traits of these Fungi.
From the rumen fluid of AL-Restaki and AL-Karadi of cows, the was isolated. Using the traditional approach and the ID-Yst card system Vitek 2. A semiconductor laser system with a power of 50 mW and a wavelength of 450 nm was used in the experiment to evaluate the light laser irradiation effects on the culture growth of directly under two light irradiation conditions of 30 and 60 minutes.
According to traditional methods and the ID-Yst card system Vitek 2, predominated 7/30 (23.3%), and these strains effectively grow on medium sabouraued dextrose agar as evidenced by the carotenoid pigments that gave their colonies a salmon-pink to coral-red. Compared with a control group where no laser was used, the impact of light laser irradiation was assessed 24 hours after the irradiation using biomass (dry weight measuring yeast cell content in suspension) and microscopic analysis using Gram stain. Microscopic examinations showed the irregular shape of the cells linked to one another. The irradiated subculture of on Sabouraued dextrose agar and incubation at 37°C for 3 days demonstrated inhibited growth in 4/7 (57.1%) isolates. In addition, there was no discernible difference vertically at < 0.05 between the control group and the biomass concentration under light irradiation circumstances (30 and 60 minutes).
This study proved that is found in the rumen fluid of cows. Also, the isolated displayed sensitivity to laser irradiation lights, revealing the more significant topographical alterations of the cell structure that had happened, the irregular shape of the cells, and how they were connected as a result of evolution.
真菌种类广泛存在于空气中，被认为是人类皮肤、肺部、尿液和粪便的天然寄居者。因此， 、 和 是最常见的三种真菌。
本研究旨在从摩苏尔省奶牛的瘤胃液中分离 ，并确定激光光照射如何影响这些真菌的生长和形态特征。
从 AL-Restaki 和 AL-Karadi 奶牛的瘤胃液中分离出 。使用传统方法和 ID-Yst 卡系统 Vitek 2，使用功率为 50mW、波长为 450nm 的半导体激光系统，在两种光照条件下（30 分钟和 60 分钟）直接评估激光光照射对 的培养生长的影响。
根据传统方法和 ID-Yst 卡系统 Vitek 2， 占 7/30（23.3%），这些菌株有效地在萨布罗德葡萄糖琼脂培养基上生长，这可以从其菌落的类胡萝卜素色素看出，其颜色为鲑鱼粉红色至珊瑚红色。与未使用激光的对照组相比，在照射后 24 小时使用生物量（测量悬浮液中酵母细胞含量的干重）和革兰氏染色的显微镜分析评估光激光照射的影响。显微镜检查显示细胞彼此不规则地连接。在萨布罗德葡萄糖琼脂上的辐照亚培养物和在 37°C 下孵育 3 天，显示在 4/7（57.1%）分离株中生长受到抑制。此外，在光照条件下（30 分钟和 60 分钟），对照组和生物量浓度之间在垂直方向上没有明显差异（ < 0.05）。
本研究证明 在奶牛的瘤胃液中被发现。此外，分离出的 对激光辐射光表现出敏感性，显示出细胞结构发生的更显著的形貌变化，细胞的不规则形状以及它们如何因进化而连接。
