Effect of laser radiation on the growth of isolated from rumin fluid of cows in the Nineveh, Iraq.

BACKGROUND

The fungi species are widespread airborne contaminants and are thought to be natural occupants of human skin, lungs, urine, and feces. Therefore, , , and are three of the most prevalent species.

AIM

This study aims to isolate from the rumen fluid of cows in the province of Mosul and to determine how laser light irradiation affects the growth and morphological traits of these Fungi.

METHODS

From the rumen fluid of AL-Restaki and AL-Karadi of cows, the was isolated. Using the traditional approach and the ID-Yst card system Vitek 2. A semiconductor laser system with a power of 50 mW and a wavelength of 450 nm was used in the experiment to evaluate the light laser irradiation effects on the culture growth of directly under two light irradiation conditions of 30 and 60 minutes.

RESULTS

According to traditional methods and the ID-Yst card system Vitek 2, predominated 7/30 (23.3%), and these strains effectively grow on medium sabouraued dextrose agar as evidenced by the carotenoid pigments that gave their colonies a salmon-pink to coral-red. Compared with a control group where no laser was used, the impact of light laser irradiation was assessed 24 hours after the irradiation using biomass (dry weight measuring yeast cell content in suspension) and microscopic analysis using Gram stain. Microscopic examinations showed the irregular shape of the cells linked to one another. The irradiated subculture of on Sabouraued dextrose agar and incubation at 37°C for 3 days demonstrated inhibited growth in 4/7 (57.1%) isolates. In addition, there was no discernible difference vertically at < 0.05 between the control group and the biomass concentration under light irradiation circumstances (30 and 60 minutes).

CONCLUSION

This study proved that is found in the rumen fluid of cows. Also, the isolated displayed sensitivity to laser irradiation lights, revealing the more significant topographical alterations of the cell structure that had happened, the irregular shape of the cells, and how they were connected as a result of evolution.