Ladoke Akintola University of Technology, Department of Medical Microbiology and Parasitology.
Babcock University, Department of Pharmacology.
Afr Health Sci. 2023 Mar;23(1):765-773. doi: 10.4314/ahs.v23i1.80.
This study investigated the genetic diversity of among asymptomatic pregnant women on intermittent preventive treatment with sulfadoxine-pyrimethamine (IPTp-Sp) in Osogbo, southwest Nigeria. Blood sample was obtained from consenting pregnant women attending antenatal clinics. Microscopy and Polymerase chain reaction (PCR) were employed to diagnose and analyse genetic diversity. Of the 301 samples, 53 (18%) and 83 (28%) were positive for by microscopy and PCR, respectively. Using the merozoite surface protein (, and glutamate-rich protein (glurp) genes of as polymorphic markers, the msp-1 gene showed nine alleles with R033 (66.7%) being predominant, followed by K1 (45.5%) and MAD20 (33.3%). The msp-2 gene had 16 alleles (eight each for FC27 and 3D7). The 3D7 alleles (82.1%) was significantly more than FC27 alleles (48.2%) (p = 0.0093). Nine alleles were detected with glurp gene, presenting with the highest monoclonal and the lowest polyclonal infection. The multiplicity of infection (MOI) of 1.5, 1.8, and 1.2 were obtained for , and genes. In light of the high genetic diversity among pregnant women on IPT-Sp in this study, additional strategies for preventing and controlling malaria in pregnancy might be required.
本研究调查了尼日利亚西南部奥索戈博无症状孕妇在间歇性预防治疗中使用磺胺多辛-乙胺嘧啶（IPTp-Sp）时的基因多样性。从同意参加产前检查的孕妇中采集血样。采用显微镜检查和聚合酶链反应（PCR）来诊断和分析遗传多样性。在 301 个样本中，显微镜检查和 PCR 分别有 53（18%）和 83（28%）呈阳性。使用裂殖子表面蛋白（msp-1、msp-2 和谷氨酸丰富蛋白（glurp）基因作为多态性标记，msp-1 基因显示有 9 个等位基因，R033（66.7%）为主导，其次是 K1（45.5%）和 MAD20（33.3%）。msp-2 基因有 16 个等位基因（FC27 和 3D7 各 8 个）。3D7 等位基因（82.1%）明显多于 FC27 等位基因（48.2%）（p = 0.0093）。glurp 基因检测到 9 个等位基因，呈现出最高的单克隆和最低的多克隆感染。感染程度（MOI）为 1.5、1.8 和 1.2，分别对应 、 和 基因。鉴于本研究中接受 IPTp-Sp 的孕妇中存在较高的 基因多样性，可能需要采取额外的策略来预防和控制孕期疟疾。
