Naaz Sheeba, Ahmad Nadeem, Jameel M Rizwan, Al-Huqail Asma A, Khan Faheema, Qureshi M Irfan
Department of Biotechnology, Faculty of Natural Sciences, Jamia Millia Islamia (A Central University), New Delhi 110025, India.
Department of Biosciences, Faculty of Natural Sciences, Jamia Millia Islamia (A Central University), New Delhi 110025, India.
ACS Omega. 2023 Jul 19;8(30):27597-27611. doi: 10.1021/acsomega.3c03325. eCollection 2023 Aug 1.
In plants, ATP-binding cassette (ABC) transporters facilitate the movement of substrates across membranes using ATP for growth, development, and defense. Soils contaminated with toxic metals such as cadmium (Cd) and mercury (Hg) might adversely affect the metabolism of plants and humans. In this study, a phylogenetic relationship among soybeans' () ATP binding cassette (GmABCs) and other plant ABCs was analyzed using sequence information, gene structure, chromosomal distribution, and conserved motif-domain. The ontology of GmABCs indicated their active involvement in trans-membrane transport and ATPase activity. Thirty-day-old soybean plants were exposed to 100 μM CdCl and 100 μM HgCl for 10 days. Physiological and biochemical traits were altered under stress conditions. Compared to Control, GmABC transporter genes were differentially expressed in response to Cd and Hg. The qRT-PCR data showed upregulation of seven ABC transporter genes in response to Cd stress and three were downregulated. On the other hand, Hg stress upregulated four GmABC genes and downregulated six. It could be concluded that most of the ABCB and ABCG subfamily members were actively involved in heavy metal responses. Real-time expression studies suggest the function of specific ABC transporters in Cd and Hg stress response and are helpful in future research to develop stress-tolerant varieties of soybean.
在植物中，ATP结合盒（ABC）转运蛋白利用ATP促进底物跨膜运输，以支持植物的生长、发育和防御。被镉（Cd）和汞（Hg）等有毒金属污染的土壤可能会对植物和人类的新陈代谢产生不利影响。在本研究中，利用序列信息、基因结构、染色体分布和保守基序结构域，分析了大豆（）ATP结合盒（GmABCs）与其他植物ABC转运蛋白之间的系统发育关系。GmABCs的本体论表明它们积极参与跨膜运输和ATP酶活性。将30日龄的大豆植株暴露于100μM CdCl和100μM HgCl中10天。在胁迫条件下，生理和生化特性发生了改变。与对照相比，GmABC转运蛋白基因在响应Cd和Hg时差异表达。qRT-PCR数据显示，7个ABC转运蛋白基因在Cd胁迫下上调，3个下调。另一方面，Hg胁迫上调了4个GmABC基因，下调了6个。可以得出结论，ABCB和ABCG亚家族的大多数成员都积极参与重金属响应。实时表达研究表明了特定ABC转运蛋白在Cd和Hg胁迫响应中的功能，有助于未来培育耐胁迫大豆品种的研究。
